Microscopy Flashcards
Which microscope has the higher magnification? Light or electron?
Electron microscope
Light microscope [500x - 1500x]
electron microscope [16000x - 1000000x]
What is meant by resolution?
the ability to distinguish between 2 points
What is the function of the stage clips?
To hold the slide in place on the stage
What is the equation to calculate the actual size of a cell?
Magnification = image size / actual size
What is the primary function of a microscope?
To magnify small objects so they can be seen in detail.
True or False: Light microscopes use beams of electrons to magnify specimens.
False [that describes the electron microscope]
Fill in the blank: The part of the microscope that holds the slides in place is called the ______.
stage [and has stage clips to secure it in place]
What is the total magnification if the objective lens is 10x and the eyepiece lens is 20x?
200x
Which type of microscope uses visible light to illuminate samples?
Light microscope
Identify one advantage of using an electron microscope over a light microscope.
Higher resolution and magnification.
What is the purpose of the diaphragm in a microscope?
To control the amount of light that reaches the specimen.
Multiple Choice: Which part of the microscope is adjusted to bring the specimen into focus?
A) Coarse adjustment knob
B) Fine adjustment knob
C) Both A and B
C) Both A and B
What is the name of the lens closest to the specimen?
Objective lens
True or False: The higher the magnification, the lower the resolution.
False
What is the purpose of preparing a slide for microscopy?
To allow for the observation of small specimens under a microscope.
True or False: A cover slip should be placed on top of the specimen after adding a drop of water.
True
Fill in the blank: The __________ is used to hold the specimen in place on the slide.
coverslip
Multiple Choice: Which of the following is NOT a step in preparing a microscope slide?
A) Adding a drop of dye onto the sample
B) Placing a cover slip
C) Heating the slide
D) Labeling the slide
C) Heating the slide