Microscopy

Question 1

What is the function of a stage micrometer

Answer 1

• to calibrate an eyepiece graticule so it can be used to make measurements

Question 2

How is an eyepiece graticule calibrated

Answer 2

• select the desired magnification and objective
• place the stage micrometer of stage
• line up the scales of the micrometer and the eyepiece graticule
• count the number of division on the eyepiece graticule equivalent to each division on the stage micromete
• calculate the length of one division of the eyepiece graticule

Question 3

Outline procedure preparing an onion slide

Answer 3

• use forceps to peel epidermal layers off the onion
• mount onto slide with a drop of water
• add drop of iodine solution on the slide
• lower the cover slip onto the miscrope slide using a mounted needle slowly prevent air bubbles from forming

Question 4

Outline procedure of focusing with a light micrscope

Answer 4

• rotate the revolving nosepiece to line up the lower power objective len
• look throguh the eyepiece and use coarse adjustment knob to lower tha stage to focus the image
• ue the fine adjustment knob in order to focus image further