DNa extraction Flashcards
1
Q
Why are strawberries used for this practical
A
- large genome
- soft and hence can be broken down easily
2
Q
Purpose of adding washing up liquid
A
break down the phospholipid membranes
3
Q
Why is pineapple juice added
A
contains protease to digest proteins in cells
4
Q
Why is salt added
A
neutralise the negative charge of phosphates in DNA lowering its solubulity to be precipitated
5
Q
Why is the mixture heated at 60 degrees celcius
A
speed up rate of reaction of the proteases in pineapple so cell proteins are digestes at a faster rate
6
Q
Why is iced ethanol added
A
the solubility of DNa is lowed in ethanol and cold temperatures allow DNA to be precipitated out
