Microscopy Flashcards
Laboratory equipment designed to magnify organisms, materials and other samples that minuscule to be observed under the normal vision.
MICROSCOPES
GENERAL PROPERTIES OF MICROSCOPE
RESOLUTION
MAGNIFICATION
CONTRAST
SENSITIVITY
GENERAL PROPERTIES OF MICROSCOPE
- Crispness of the image (Detailed appearance )
RESOLUTION
GENERAL PROPERTIES OF MICROSCOPE
- Closeness of the image (Distance-related appearance)
MAGNIFICATION
GENERAL PROPERTIES OF MICROSCOPE
- Distinguishable components (Equal clarify of each section)
CONTRAST
GENERAL PROPERTIES OF MICROSCOPE
- Easy manipulation for adjustments (Gradual changes)
SENSITIVITY
Diverse which can be utilized depending on the required magnification power and observed specimen of interest.
MICROSCOPES
CLASSIFICATION OF MICROSCOPE
COMPOUND LIGHT
DISSECTING/ STEREO
ELECTRON
CLASSIFICATION OF MICROSCOPE
- Basic microscope (mono-ocular or binocular)
COMPOUND LIGHT
CLASSIFICATION OF MICROSCOPE
- Free-area microscope (Moving or living specimen)
DISSECTING / STEREO
CLASSIFICATION OF MICROSCOPE
- Ultramicroscopic magnification (Single molecule)
ELECTRON
3 CATEGORIZED STRUCTURES OF MICROSCOPE
BODY
STAGE
OPTICS
STRUCTURES OF MICROSCOPE
- Connects the stage, optics, and electrical components.
BODY
STRUCTURES OF MICROSCOPE
- Platform for specimen preparation and manipulation.
STAGE
STRUCTURES OF MICROSCOPE
- Observation and magnification of specimen.
OPTICS
COMPONENTS OF THE BASE
BASE
ARM
LIGHT SOURCE
LIGHT ADJUSTMENT KNOB
LIGHT SWITCH
COMPONENTS OF THE BASE
- Supports the entire weight of the microscope and it’s components.
BASE
COMPONENTS OF THE BASE
- Connects the body to the other structures of microscope.
ARM
COMPONENTS OF THE BASE
- Provides uniform and stable supply of light.
LIGHT SOURCE
COMPONENTS OF THE BASE
- Adjusts the supply of light.
LIGHT ADJUSTMENT KNOB
COMPONENTS OF THE BASE
- Allows electrical supply to the light source.
LIGHT SWITCH
COMPONENTS OF THE STAGE
STAGE
STAGE ADJUSTMENT KNOB
STAGE CLIPS
IRIS DIAPHRAGM
CONDENSER
SLIDES
COVERSLIP
COURSE ADJUSTMENT KNOB
FINE ADJUSTMENT KNOB
COMPONENTS OF THE STAGE
- Serves as a platform in which the sample is placed during observation.
STAGE
COMPONENTS OF THE STAGE
- Adjusts the position of the sample in the stage.
STAGE ADJUSTMENT KNOB
COMPONENTS OF THE STAGE
- Hold the sample securely in the stage during observation.
STAGE CLIPS
COMPONENTS OF THE STAGE
- Allows straight passage of light to the sample in the stage.
IRIS DIAPHRAGM
COMPONENTS OF THE STAGE
- Concentrates the rays of light to a single point of entry.
CONDENSER
COMPONENTS OF THE STAGE
- 3”x 1”x1” Rectangular glass that fixes the sample in a singular level.
SLIDES
COMPONENTS OF THE STAGE
- 22mm Square glass that wedges the sample into the glass slide.
COVERSLIP
COMPONENTS OF THE STAGE
- Allows massive vertical movement of the stage.
COURSE ADJUSTMENT KNOB
COMPONENTS OF THE STAGE
- Allows minimal vertical movement of the stage.
FINE ADJUSTMENT KNOB
COMPONENTS OF THE OPTICS
EYE-PIECE
BODY TUBE
REVOLVING NOSE-PIECE
OBJECTIVES
COMPONENTS OF THE OPTICS
- Portal of vision for the user observation.
EYE-PIECE
COMPONENTS OF THE OPTICS
- Holds glass that has 10x or 20x magnification for initial view.
BODY TUBE
COMPONENTS OF THE OPTICS
- Rotating platform that holds multiple objective lens.
REVOLVING NOSE-PIECE
COMPONENTS OF THE OPTICS
- Specific glass lens with differential magnification levels.
OBJECTIVE LENS
TYPES OF OBJECTIVE LENS
SCANNER (4x)
LOW POWER OBJECTIVE (10x)
HIGH POWER OBJECTIVE (40x)
OIL IMMERSION OBJECTIVE (100x)