Microscopy

Question 1

Match the type of microscopy to its definition:

__________: use optics and white light to contrast object
Light microscope and background
______________: localizes tagged proteins on cells
_______________: allows study of cells movements
________________: higher resolution
________________: acquired by computer
__________________: electron as source of investigating
illumination radiation

A. Electron microscopy B. Confocal microscopy C. Live microscopy D. Digital microscopy E. Fluorescence F. Bright Field

Answer 1

• Bright field: use optics and white light to contrast object Light microscope and background
• fluorescence: localizes tagged proteins on cells
• Live microscopy: allows study of cells movements

• Confocal microscopy: higher resolution

• Digital microscopy: acquired by computer

• Electron microscopy: electron as source of investigating
illumination radiation

Question 2

Match the objects to the microscope you would use:

1. Plant and animal cells,piece of strong 1mm-1micrometre
2. Proteins, lipids, atoms

Light microscope and Electron microscope

Answer 2

Light microscope = Plant and animal cells,piece of strong 1mm-1micrometre
Electron microscope = Proteins, lipids, atoms

Question 3

What is the formula for overall magnification

Answer 3

ocular lens (eyepiece lens) x objective lens (microscope embedded lens) 10X x 40X = 400X

Question 4

Match these definitions to their terminology

_____________: amount of section visualized on eyepiece.
______________: is the clearness of the plane of focus on
field of view
____________: distance of objective from specimen

A. Field of view B. Working distance C. Depth of field

Answer 4

Field of view: amount of section visualized on eyepiece.

Depth of field : is the clearness of the plane of focus on
field of view, is one thing in focus or the whole field of view in focus

Working distance: distance of objective from specimen

Question 5

Define what primary cells are

To prepare primary culture:
1. _________________________ must be broken
2. Tissue fragments are treated with___________ (depletes_________ on CAMS)
3. Cells are placed in _______________ in dishes

Answer 5

Primary cells = cells isolated directly from tissues

To prepare primary culture:
1. Cell-cell and cell-matrix interactions must be broken
2. Tissue fragments are treated with protease (depletes Calcium on CAMS)
3. Cells are placed in nutrient rich,medium in dishes

Question 6

Define what a cell strain is

Answer 6

lineage of cells originating from one initial primary culture

Question 7

Match the definition to the term

__________________ cells isolated directly from tissues
To prepare primary culture

______________ cell lineage originating from one initial primary culture

__________________a culture of cells with an indefinite life span is immortal

A.Cell line B. Primary cells C. Cell strain

Answer 7

PRIMARY CELLS cells isolated directly from tissues
To prepare primary culture

CELL STRAIN cell lineage originating from one initial primary culture

CELL LINE a culture of cells with an indefinite life span is immortal

Question 8

What is the term for these definitions

_____________Ability to distinguish (separate) two objects situated close together

_____________ difference in light intensity between the specimen image and the adjacent background

Answer 8

RESOLUTION Ability to distinguish (separate) two objects situated close together

CONTRAST difference in light intensity between the specimen image and the adjacent background

Question 9

What samples are best for viewing with immersion oil?

Answer 9

1. Dead or immobile samples