Microscopy

Question 1

What’s the maximum magnification of a light microscope?

Answer 1

x1500

Question 2

How does a light microscope produce an image?

Answer 2

Relies on the visible part of the electromagnetic spectrum.

Question 3

Are the images on a light microscope in colour?

Answer 3

Yes

Question 4

Are the images on a light microscope 2D or 3D?

Answer 4

2D

Question 5

What is the resolution of a light microscope?

Answer 5

200nm (limited)

Question 6

What is the maximum magnification on a Scanning Electron Microscope?

Answer 6

x2 million

Question 7

How does a scanning electron microscope produce an image?

Answer 7

Uses beams of electrons, scans the surface of the sample.

Question 8

Are the images of a scanning electron microscope in colour?

Answer 8

No

Question 9

Are the images on a scanning electron microscope 2D or 3D?

Answer 9

3D

Question 10

What is the resolution of a scanning electron microscope?

Answer 10

0.4nm

Question 11

What is the maximum magnification of a transmission electron microscope?

Answer 11

x50 million

Question 12

How does a transmission electron microscope produce an image?

Answer 12

uses a beam of electrons which pass through the sample.

Question 13

Are the images on a transmission electron microscope in colour?

Answer 13

no

Question 14

are the images on a transmission electron microscope 2D or 3D

Answer 14

3D

Question 15

what is the resolution of the images on a transmission electron microscope?

Answer 15

> 0.4nm

Question 16

What are the limitations of Electron Microscopes

Answer 16

> the samples must be in a vacuum (the sample will be dead)
the specimens must be stained.
TEM sample needs to be thin so the electron can pass through.
SEM has a lower resolving power than TEM, however both have a higher resolving power than a light microscope
the images are in black and white.

Question 17

Who developed the microscope in 1590 and what did they do?

Answer 17

Hans and Jansen, they created the compound microscope, it was magnified but the images were blurry.

Question 18

Who developed the microscope in 1665 and what did they do?

Answer 18

Hooke, developed the compound microscope and described the cells in a cork.

Question 19

Who developed the microscope in 1673 and what did they do?

Answer 19

Anton Van Leewenhoek, without knowing it he observed the first living cells.

Question 20

Who developed the microscope in 1833 and what did they do?

Answer 20

Robert Brown, discovered the cell nucleus.

Question 21

Who developed the microscope in 1838 and what did they do?

Answer 21

Matthios Jakob, worked with another scientist, then compared plant and animal cells and made the cell theory.

Question 22

What is the cell theory?

Answer 22

The cell theory has 3 tenants:
-all living organisms are made of one or more cells.
-cells are the basic structural units of all organisms
-all cells originate from pre-existing cells

Question 23

Who developed the microscope in 1852 and what did they do?

Answer 23

Robert Remak, observed cell division, however his theory wasn’t accepted by society until after 1855

Question 24

Who developed the microscope in 1860 and what did they do?

Answer 24

Louss Pasteur, disproved spontaneous generation.