microscopy Flashcards

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1
Q

what is used to magnify and see specimens what the naked eye cannot see

A

microscope

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2
Q

who discovered the microscope

A

ZACHARIAS JANSSEN

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3
Q

who is the father of the microscope?

A

ANTONI VAN LEEUWENHOEK

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4
Q

microscope with one lens, Similar to this will be a Magnifying lens but we don’t call this a microscope because it is only a lens

A

SIMPLE MICROSCOPE

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5
Q

two or more lens,

A

COMPOUND LIGHT MICROSCOPE

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6
Q
  • Has space for your hands to move
  • It doesn’t have a stage
  • light source at the top and bottom
A

STEREOMICROSCOPE

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7
Q

what allows u to see whatever is in here and to see whatever is in focus thus allowing you to trace viewed specimens

A

drawing tube

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8
Q

microscope that is only used by experts and depends on electron beams to show you images

A

electron microscopy

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9
Q

allows you to view the surface

A

sem image or scanning electron microscope

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10
Q

shows you which structure of the specimen is more dense than the other, allows u to differentiate or identify the different parts or the internal structures of the specimen

A

tem image or transmission electron microscope

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11
Q

this is the real 3d, allows u to view surface and internal structures

A

atomic force microscopy

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12
Q

similar to SEM image but instead of electron, it used laser. When you target a certain structure, you could select a certain die, subject it to the specimen, and view it under this microscope

A

confocal microscopy

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13
Q

it is where u view the specimen and is 10x

A

eyepiece

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14
Q

has a scanner that is 4x, LPO, HPO, AND OIO

A

Objective lenses

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15
Q

Low power objective =

A

10x

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16
Q

high power objective =

A

40x

17
Q

oil immersion objective =

A

100 x

18
Q

it can revolve or rotate and lets u view the specimen in diff. magnification

A

nose piece

19
Q

this is used when there are different or multiple lenses

A

total magnification

20
Q

how do u get the total magnification?

A

eyepiece mag. x objective mag.

21
Q

Where the specimen is placed
where the unknown length is

A

stage

22
Q

keeps specimen in place

A

stage clips/holder

23
Q

connects the eyepiece and nose piece

A

arm

24
Q

supports the whole microscope

A

base

25
Q

elevates and lifts, moves the stage, is bigger than other knob

A

coarse adjustment knob

26
Q

makes u zoom in and out, is the smaller knob

A

fine adjustment knob

27
Q

can reflect and gives u light. Makes the light pass through the condenser

A

light source / mirror

28
Q

it condenses light and make it much more brighter
- When you have higher magnification, the light should be much more focused.

A

condenser

29
Q

the bg is bright or white

A

white filmthe

30
Q

the bg is dark

A

black film

31
Q

we see this in electron microscope because it shows u which is much more darker

A

face contrast

32
Q

-placed directly on the eyepiece
-Known value, needs calibration
- it will look the same for all magnification

A

ocular micrometer

33
Q

placed on the stage

-Larger magnification = decrease in spaces
-Smaller magnification = increase in spaces
-can be magnified depending on the objective lens

A

stage micrometer

34
Q

computation of calibration factor

A

CF = (Sm) (10m) / (0m)

35
Q

computing for actual size

A

(0m)(CF)

36
Q

actual size ocular

A

number of spaces X CF