microscopy Flashcards
what is used to magnify and see specimens what the naked eye cannot see
microscope
who discovered the microscope
ZACHARIAS JANSSEN
who is the father of the microscope?
ANTONI VAN LEEUWENHOEK
microscope with one lens, Similar to this will be a Magnifying lens but we don’t call this a microscope because it is only a lens
SIMPLE MICROSCOPE
two or more lens,
COMPOUND LIGHT MICROSCOPE
- Has space for your hands to move
- It doesn’t have a stage
- light source at the top and bottom
STEREOMICROSCOPE
what allows u to see whatever is in here and to see whatever is in focus thus allowing you to trace viewed specimens
drawing tube
microscope that is only used by experts and depends on electron beams to show you images
electron microscopy
allows you to view the surface
sem image or scanning electron microscope
shows you which structure of the specimen is more dense than the other, allows u to differentiate or identify the different parts or the internal structures of the specimen
tem image or transmission electron microscope
this is the real 3d, allows u to view surface and internal structures
atomic force microscopy
similar to SEM image but instead of electron, it used laser. When you target a certain structure, you could select a certain die, subject it to the specimen, and view it under this microscope
confocal microscopy
it is where u view the specimen and is 10x
eyepiece
has a scanner that is 4x, LPO, HPO, AND OIO
Objective lenses
Low power objective =
10x
high power objective =
40x
oil immersion objective =
100 x
it can revolve or rotate and lets u view the specimen in diff. magnification
nose piece
this is used when there are different or multiple lenses
total magnification
how do u get the total magnification?
eyepiece mag. x objective mag.
Where the specimen is placed
where the unknown length is
stage
keeps specimen in place
stage clips/holder
connects the eyepiece and nose piece
arm
supports the whole microscope
base
elevates and lifts, moves the stage, is bigger than other knob
coarse adjustment knob
makes u zoom in and out, is the smaller knob
fine adjustment knob
can reflect and gives u light. Makes the light pass through the condenser
light source / mirror
it condenses light and make it much more brighter
- When you have higher magnification, the light should be much more focused.
condenser
the bg is bright or white
white filmthe
the bg is dark
black film
we see this in electron microscope because it shows u which is much more darker
face contrast
-placed directly on the eyepiece
-Known value, needs calibration
- it will look the same for all magnification
ocular micrometer
placed on the stage
-Larger magnification = decrease in spaces
-Smaller magnification = increase in spaces
-can be magnified depending on the objective lens
stage micrometer
computation of calibration factor
CF = (Sm) (10m) / (0m)
computing for actual size
(0m)(CF)
actual size ocular
number of spaces X CF
