Microscopy

Question 1

What is magnification?
what is resolution?

Answer 1

magnification -how many times bigger
an image is compared to actual object size
Resolution - the ability to see two objects as separate entities (detail)

Question 2

How do you calculate magnification ?

Answer 2

magnification:
size of image /size of real object

Question 3

how do you do magnification calculation with a scale bar?

Answer 3

-Find magnification of scale bar by dividing the ‘ruler measurement’ by the size given of scale bar
-Then use that info with the formula triangle :
I : AxM

Question 4

How do youconvert
from mm to um to nanometres?

Answer 4

x1000. x1000
mm > um > nm
/1000. /1000

Question 5

Describe a light microscope

Answer 5

• light is sent from light source through a specimen, magnified by glass lenses
  *living cells
• Black and white image.
• Cheap
• easy to use
• 2D image
• convex lenses, long wavelength

Question 6

Deschbe a TEM microscope

Answer 6

•electrons pass through specimen to create image
• 2D
• Black and White
• specimen stained + in vaccum
• High magnification and resolution

Question 7

Describe an SEM microscope

Answer 7

• electrons bounce off surface of specimen
•3D
• black+ white but can false colour with computer
• High magnification and resolution

Question 8

Describe a Laser scanning confocal microscope

Answer 8

• uses a high power beam of light
to create mage. (laser)
• 3D and 2D
• fluorescent
• expensive
•high resolution

Question 9

What are the resolutions of
• light
•SEM
•TEM
microscopes ?

Answer 9

•light: 200
•SEM : 3 - 10
•TEM : 0.2 - 0.5

Question 10

what are the magnifications of
- light
-SEM
- TEM mioroscopes ?

Answer 10

•light: x 1,500 - 2000
•SEM: x 100,000 - 500,000
•TEM: x 500,000 - 2,000,000