Eyepiece graticule and microscope parts

Question 1

How do you calibrate an eyepiece graticule?

Answer 1

• PIace micrometer on stage + focus
  microscope
• line up micrometer with eyepiece
  graticule
  -find scale e.g 50 eyepiece graticule =1mm so
  1 eyepiece graticule = 1/50
  -for each magnification calibrate the eyepiece again

Question 2

why cant eyepiece graticules be used directly to measure the size of object?

Answer 2

-each objective lens will magnify to a different degree
-graticule must be calibrated each time

Question 3

What are the first Six parts of a light microscope?

Answer 3

1. Eyepiece -lens magnifies by 10x
2. Body tube - separates eyepiece from objective lenses
3. Revolving nose piece - holds and rotates all objective lenses
4. objective lenses - three low medium and high magnifying lenses 10-40x
5. Arm - supports body tube
6. Stage - supports slide being used

Question 4

What are the last six parts of a light microscope?

Answer 4

7. Stage clips - hold slide in place
8. Diaphragm - controls amount of light
9. Corse adjustment knob - allows quick focusing by moving stage up and down
10. Fine adjustment knob - focusing finer details of specimen being used
11. Light source
12. Base - supports the miscroscope