Microscopy Flashcards

1
Q

Function of microscope.

A

Enlarge the image of objects too small to be seen by the naked eyes.

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2
Q

Base of microscope

A

Bottom of microscope, for support.

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3
Q

Eyepiece (Ocular lens)

A

To see the image of specimen.

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4
Q

Body tube

A

Connect eyepiece to the objective lenses.

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5
Q

Revolving nosepiece

A

Holds 2 or more objective lenses AND can rotated to easily change power.

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6
Q

Stage

A
  • flat platform
  • place the slides.
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7
Q

Iris/ diaphragm

A

controls the light going through the aperture.

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8
Q

Condenser

A

Collect and focus the light from illuminator to the specimen.

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9
Q

Objective lenses

A
  • gathers light from specimen
    -** focused to produce real image** that is seen on the ocular lenses.
  • increase the magnification of the specimen.
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10
Q

Stage clips

A

hold the slide in place.

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11
Q

Light

function of light in microscope.

A

provide light to view specimen.

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12
Q

Coarse adjustment knob

A
  • large, round knob
  • used for focusing the specimen
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13
Q

Fine adjustment knob

A
  • small, round knob
  • to fine-tune the focus of your specimen.
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14
Q

Coaxial stage controls (stage controls)

A

Allow the slide to move to right and left, back and forth.

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15
Q

Why there is a concave slide

A

Designed to hold few drops of liquid.

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16
Q

How immersion oil increses the resolving power of microscope?

A

By replacing the air gap between the immersion objective lens and cover glass with a high refractive index medium and reducing light refraction.

17
Q

Definition of Magnification

A

Ratio of an object’s size to its real size.

18
Q

Meaning of Resolution

A

A measure of the clarity of the image.

The amount of fine detail that can be seen.

19
Q

What is Enhanced resolution?

A

Ability to observe 2 nearby objects as distinct objects.