Aseptic Technique

Question 1

Definiton of Aseptic Technique

Answer 1

• method that involves target-specific practices and procedures under** suitably controlled conditions** to reduce the contamination from microbes.
• performed under sterile conditions.

Question 2

Objectives of aseptic technique

Answer 2

1. acquire knowledge of aseptic technique.
2. avoid the contamination of cultures
3. subculture.
4. isolate pure culture.
5. inhibit lab microbes from being distributed.

Question 3

Tools for maintaining aseptic condition

Answer 3

1. Bunsen burner
2. The Laminar flow unit

Question 4

Purpose of bunsen burner in maintaining aseptic conditions

Answer 4

• open flame is to create a cone of hot air above, to reduce the viability of organisms on suspended dust particles.
• sterilizing inoculating loops, warming glass bottle necks, igniting alcohol.

However, it is not practical in some situations ( laminar flow unit )

Question 5

Purpose of laminar flow unit.

Answer 5

Can further help prevent contamination of reagents and biological cultures.

Question 6

Elements of aseptic techniques microbiology.

Answer 6

1. Sterile handling
2. Sterile work area
3. Sterile reagents and media
4. Good personal hygiene.

Question 7

Example of sterile work area

Answer 7

1. work surface, surrounding areas, equipment should disinfected thoroughly before and after use.
2. Wipe the work surface with 70% ethanol before and during work.

Question 8

Examples of good personal hygiene

Answer 8

1. Wash hands before and after working with cell cultures.
2. Wearing personal protective equipment.

reduce probability of contamination from shed skin, dirt and dust.

Question 8

Examples of good personal hygiene

Answer 8

1. Wash hands before and after working with cell cultures.
2. Wearing personal protective equipment.

reduce probability of contamination from shed skin, dirt and dust.

Question 9

Tools for sterilizing reagents and media.

Answer 9

• Autoclave
• sterile filter

Question 10

Example of sterile handling

Answer 10

• use sterile glass/ disposable plastic pipette and pipettor to work with liquids.
• use each pipette only once to avoid cross contamination.
• Do not unwrap sterile pipettes until ready to use.
• Always cap the bottles and flasks after use.

Question 11

What is biosafety?

Answer 11

Addressing of the safe handling and containment of transferable microorganisms and unwarranted biomaterials.