Culture Medium and Culture Method Flashcards
Need for Culture media
they have to be grown separately on culture media and obtained as pure culture for study.
Types of culture medium
- Liquid medium
- Solid medium
Why solid medium is prefered to study microbes?
- distinct colony morphology
- characteristics - easy to identify
Types of culture media
based on CIO
- Consistency
- Constituents/ ingredients
- Oxygen requirement
Types of consistency
of culture media
cair, keras, separuh keras
- Solid medium
- Liquid medium
- Semi solid medium
examples of each consistency media
example of solid, liquid and semi solid
- Nutrient agar, blood agar ( solid )
- nutrient broth ( liquid )
- motility medium ( semi solid )
Types of ingredients of media
- Simple medium
- Complex medium
- Synthetic / defined medium
- Special medium
Purposes of culture methods
- Isolate bacteria in pure cultures.
- Demonstrate their properties.
- Maintain stock cultures.
- Obtain sufficient growth for preparation of antigens and other tests.
employed depend on the purpose for which they are intend
Culture methods include:
- Streak culture
- Lawn culture
- Stroke culture
- Stab culture
- Pour plate method
- Liquid culture
- Anaerobic culture methods
Purpose of streak culture
Why we do streaking?
Isolation of bacteria in pure culture from clinical specimens.
What equipment used for streak culture?
Platinum wire / Nichrome wire
Uses of Lawn culture
- Bacteriophage typing
- Antibiotic sensitivity typing
- Preparation of bacterial antigens and vaccines.
How Lawn culture are prepared?
By flooding the surface of the plate with a liquid suspension of the bacterium.
Function of stroke culture
Provide a pure growth of bacterium for slide agglutination and other diagnostic tests.
Uses of stab culture
- Demonstration of gelatin liquefaction.
- Oxygen requirements of the bacterium under study.
- Maintenance of stock cultures.
