Microscopy

Question 1

First observers of cells

Answer 1

Robert Hooke (1635-1703, English) and Antonio Van Leeuwenhoek (1632-1723 Dutch)

Question 2

Light Microscope

Answer 2

uses visible light that is magnified and focused as it passes through glass lenses and specimen before hitting viewers eye

Question 3

electron microscope

Answer 3

uses electron beam that is passed through magnetic lenses and projected on photographic film

Question 4

flourescent microscopy

Answer 4

uses laser light or near single wavelength of light

Question 5

compound microscope

Answer 5

2 lenses, light passes through specimen so object = thin

Question 6

stereomicroscope

Answer 6

light reflects object can be thick, 3d objects

Question 7

millimeter

Answer 7

.001

Question 8

micrometer

Answer 8

.000001

Question 9

mm to micrometers

Answer 9

1 mm = 1000 micrometers

Question 10

scope tips

Answer 10

scanning 4x, low power, 10 x, high power 40x

Question 11

resolution

Answer 11

the ability to reveal details or distinguish two objects that are close

Question 12

contrast

Answer 12

the differential absorption of light by different parts of object being viewed

Question 13

working distance

Answer 13

when image is in focus it is the distance between slide on stage and bottom of objective lens

Question 14

field of view

Answer 14

area you see when looking in microscope

Question 15

parfocal

Answer 15

image general remains in focus when moving magnification to another

Question 16

parcentric

Answer 16

image will generally remain in center of field of view

Question 17

FOV

Answer 17

4.3 x (10x, 4x, 40x) / 10x

Question 18

total magnification

Answer 18

objective lens x ocular lens, always 10 x (40, 4, 10)