Microscopy Flashcards

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1
Q

Magnification

A

How many times larger the image is than the actual size of the object being viewed.

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2
Q

Resolution

A

The ability to see individual objects as separate entities.

The Lowe the resolution, the better

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3
Q

Magnification Formula

A

Magnification=Size of image/Actual size of object.

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4
Q

Light microscope (normal)

A
  • Low resolution of 0.2 micrometers (um).
  • Maximum useful magnification of X1500.
  • Used to observe whole cells.
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5
Q

Laser scanning confocal microscopes.

A
  • Causes dye to give off light.
  • 3D image.
  • Out of focus light is blocked, producing a clear image.
  • Look at different depths in thick specimen.
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6
Q

Transmission Electron Microscope (TEM)

A
  • Maximum resolution of 0.0002um.
  • Maximum magnification of X1,000,000
  • Only 2D images.
  • Used to observe organelles.
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7
Q

Scanning electron microscope (SEM).

A
  • Maximum resolution of 0.002 um
  • Maximum magnification of X 500,000
  • Produces 3D images.
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8
Q

Reason for staining

A
  • Staining increases contrast.

- Makes it easier to recognise different organelles.

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9
Q

How to prepare a microscope slide

A
  • Use tweezers to pick up the specimen and place it in the middle of a clean slide.
  • Put a cover slip on top of the specimen.
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10
Q

How to use a Light microscope.

A
  • Clip the slide onto the stage.
  • Select the objective lens with the lowest magnification.
  • Use the Coarse adjustment knob to bring the stage up to just below the objective lens.
  • Use the coarse adjustment knob to lower the stage, away from the objective lens until the image is in focus.
  • Swap to an objective lens with a higher magnification to see the slide with a greater magnification.
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11
Q

How to use an eyepiece graticule and a state micrometer.

A
  • Line up the eyepiece graticule and the stage micrometer.
  • Each division of the stage micrometer is 0.1mm long.
  • At this magnification, 1 division of the stage micrometer=4.5 divisions on the eyepiece graticule.
  • Division on the stage micrometer/divisions on the eyepiece graticule(0.1/4.5=0.022 mm)
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