Microscopic techniques Flashcards
optical microscopy
use visible light and a system of lenses to get magnified images of small samples
400 - 700 nm in wavelength
wavelength (m)
distance from a point in a cycle to the corresponding point in the next
peak to peak, trough to trough
frequency (Hz)
number of vibrations of a given wavelength in one second
longer wavelength = lower frequency
velocity = freq x wavelength
Absorption
all light enters, nothing exits (thermal, electrical, chemical changes)
reflection
all light is turned back into the incident material
rough surface = more diffuse reflection, less specular reflection
refraction
ray bent when passing between 2 transparent materials = change in velocity
affected by incident angle and wavelength of incident ray and surface
refractive index
sin angle1 / sin angle2 = n1/n2 = v1/v2