Microscopes and Magnification

Question 1

What is magnification?

Answer 1

How many times bigger the image size is compared to the actual size

Question 2

What is the resolution?

Answer 2

The ability to determine between 2 separate points

Question 3

What is a stain used for?

Answer 3

To highlight the organelles and add contrast

Question 4

What is the equation for magnification?

Answer 4

Actual size x Magnification = Image size

Question 5

What are specimen coated with?

Answer 5

A thin layer of metal: Gold, platinum or tungsten

Question 6

What is a Transmission Electron Microscope?

Answer 6

Shoots a beam of electrons at thin slice of a sample. Gives high resolution at a thin section and its good for learning about how components inside a cell are structured internally

Question 7

What is a Scanning electron microscope?

Answer 7

It uses a focused beam of high energy electrons which reflect off the surface of structures and gives a 3D image.

Question 8

Why do electron microscopes require a vacuum?

Answer 8

Air particles would interfere with the beam of electrons so the specimen must always be dead