Microscopes Flashcards
Purpose of microscope
Used to view cells
Asses health by looking for abnormalities
Compound light microscope
Use light focussed through different lenses to form a magnified image of a specimen or object
Commonly found in science classrooms
Electron microscope
Use a beam of electrons instead of light
TEM
Transmission Electron microscope
Can magnify up to 1 500 000 times
Beam travels down column through the specimen
Cannot see living only dead
Beam of electron passes through thin slice
SEM
Provides info on surface features
Magnify up to 300 000 times
Produces 3D images
Scanning electron microscope
Electron micrograph
A photograph taken by sem or tem
Detailed info on surfa texture shape size and arrangement of materials
STM and AFM
Scanning tunneling microscope
Atomic force microscope
Produce images of molecules within cells
Understand structure and functions
First microscope magnification
20
Compound magnification
1000-2000
Micrograph
A photo take through a microscope
Shows magnified image of the specimen camera attached instead of eye piece
Resolution
The ability to distinguish between 2 objects that are very close together
Contrast purpose
Difficult to see the parts of the cell
Some pale and transparent background
Improves images
Common contrast stains
Methylene blue
Iodine
Fluorescence microscopy
Flurorescent substances added to the cells
Glows in ultraviolet light