Microscopes Flashcards

1
Q

Purpose of microscope

A

Used to view cells

Asses health by looking for abnormalities

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2
Q

Compound light microscope

A

Use light focussed through different lenses to form a magnified image of a specimen or object
Commonly found in science classrooms

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3
Q

Electron microscope

A

Use a beam of electrons instead of light

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4
Q

TEM

A

Transmission Electron microscope
Can magnify up to 1 500 000 times
Beam travels down column through the specimen
Cannot see living only dead
Beam of electron passes through thin slice

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5
Q

SEM

A

Provides info on surface features
Magnify up to 300 000 times
Produces 3D images
Scanning electron microscope

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6
Q

Electron micrograph

A

A photograph taken by sem or tem

Detailed info on surfa texture shape size and arrangement of materials

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7
Q

STM and AFM

A

Scanning tunneling microscope
Atomic force microscope
Produce images of molecules within cells
Understand structure and functions

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8
Q

First microscope magnification

A

20

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9
Q

Compound magnification

A

1000-2000

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10
Q

Micrograph

A

A photo take through a microscope

Shows magnified image of the specimen camera attached instead of eye piece

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11
Q

Resolution

A

The ability to distinguish between 2 objects that are very close together

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12
Q

Contrast purpose

A

Difficult to see the parts of the cell
Some pale and transparent background
Improves images

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13
Q

Common contrast stains

A

Methylene blue

Iodine

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14
Q

Fluorescence microscopy

A

Flurorescent substances added to the cells

Glows in ultraviolet light

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