Microscopes Flashcards

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1
Q

Describe how light microscopes work

A

Use visible light and magnifying glasses to observe small objects

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2
Q

Describe how electron microscopes work

A

Use a beam of electrons in a vacuum with a wavelength of less than 1nm to visualise the spectrum

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3
Q

Equation for magnification, size of image, size of object

A

Size of image = magnification X size of object

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4
Q

Equation for mag of objective lens, mag of eye piece, total magnification

A

Total magnification = mag of eye piece X mag of objective lens

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5
Q

Type of specimen (light)

A

Dead or alive

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6
Q

Type of specimen (electron)

A

Dead

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7
Q

Colour of image (light)

A

Coloured

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8
Q

Colour of image (electron)

A

Black and white

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9
Q

Size of machine (light)

A

Small

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10
Q

Size of machine (electron)

A

Large

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11
Q

Benefit (light)

A

Cheap

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12
Q

Benefit (electron)

A

Better magnification and resolution

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13
Q

Magnification (light)

A

X500

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14
Q

Magnification (electron)

A

X500000

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15
Q

Resolution (light)

A

200nm

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16
Q

Resolution (electron)

A

0.1nm

17
Q

Limitations (light)

A

Lower magnification and resolution

18
Q

Limitations (electron)

A

Destroys the sample