Microscopes Flashcards

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1
Q

light microscope features

A

uses beams of light to form an image as some of the light is absorbed by dense structures

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2
Q

adv of light microscopes

A

can view living specimens and use different stains like methyl blue or iodine

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3
Q

dis of light microscopes

A

visible light has a long wavelength, giving a low resolution
lower useable magnification because of the low resolution

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4
Q

electron microscope features

A

a beam of electrons creates the image as the denser parts of the structures absorbs more electrons
has two types
creates black and white images

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5
Q

SEM (scanning)

A

forms 3D images
specimen is coated in metal
high magnification but lower than TEM

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6
Q

TEM (transmission)

A

forms 2D images
higher magnification

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7
Q

laser scanning confocal (LSC)

A

a laser scans across the specimen
it scans the different depth to view different layers in the specimen
layers can then form the 3D images

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8
Q

adv of LSC

A

higher resolution than optical microscopes
can view living specimens and see movement
can focus at different depths
3D composite images formed

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9
Q

dis of LSC

A

lower resolution than electron microscopes

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10
Q

magnification

A

how many times larger an image is compared to the actual size

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11
Q

resolution

A

the smallest distance apart where two objects are seen as separate
the smaller the distance, the higher the resolution

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