Microscopes

Question 1

light microscope features

Answer 1

uses beams of light to form an image as some of the light is absorbed by dense structures

Question 2

adv of light microscopes

Answer 2

can view living specimens and use different stains like methyl blue or iodine

Question 3

dis of light microscopes

Answer 3

visible light has a long wavelength, giving a low resolution
lower useable magnification because of the low resolution

Question 4

electron microscope features

Answer 4

a beam of electrons creates the image as the denser parts of the structures absorbs more electrons
has two types
creates black and white images

Question 5

SEM (scanning)

Answer 5

forms 3D images
specimen is coated in metal
high magnification but lower than TEM

Question 6

TEM (transmission)

Answer 6

forms 2D images
higher magnification

Question 7

laser scanning confocal (LSC)

Answer 7

a laser scans across the specimen
it scans the different depth to view different layers in the specimen
layers can then form the 3D images

Question 8

adv of LSC

Answer 8

higher resolution than optical microscopes
can view living specimens and see movement
can focus at different depths
3D composite images formed

Question 9

dis of LSC

Answer 9

lower resolution than electron microscopes

Question 10

magnification

Answer 10

how many times larger an image is compared to the actual size

Question 11

resolution

Answer 11

the smallest distance apart where two objects are seen as separate
the smaller the distance, the higher the resolution