Microscopes Flashcards
1
Q
Magnification
A
ocular magnification x objective magnification
2
Q
Ocular Magnification
A
10X
3
Q
Objective Magnification
A
4X, 10X, 50X, or 100X
4
Q
Resolution
A
Clarity of an image
5
Q
Resolving Power
A
Ability to separate 2 objects in a field of view
6
Q
Numerical Aperture
A
Describes how the condenser lens concentrates and focuses the light rays from the light source.
7
Q
Limit of Resolution
A
D = _ wavelength _
NA<sub>condenser</sub> + NA<sub>objective</sub>
D = distance between 2 objects
D = 0.2 µm for our microscopes
8
Q
Parfocal
A
A lens that stays in focus when magnification is changed (e.g. when you switch from one objective to another).
9
Q
Oil Immersion
A
- Reduces light diffraction
- Maximizes numerical aperture of objective, which improves resolution
- Used for 50X and 100X objectives.
10
Q
Arrangements of Cells
A
- Clusters: Staphy
- Chains: Strepto
- Packets: Micro
