Cultures/Smears

Question 1

Purposes of Aseptic Technique

Answer 1

• To make sure we do not contaminate slides with bacteria on the materials
• So we do not contaminate ourselves or others with bacteria
• So we do not leave bacteria on surfaces or materials when we are finished

Question 2

TSB/TSA

Answer 2

Trypticase Soy Broth/Agar

Question 3

Goals in preparing a smear

Answer 3

• Cause cells to adhere to slide to prevent them from washing off during the staining process
• To ensure that cells do not shrink or become distorted
• Prepare thin smears - helps to visualize individual cells

Question 4

Heat Fixation

Answer 4

• Causes cells to adhere to slide

- Also causes cell death