Endospore Stain Flashcards
1
Q
Bacteria that form spores
A
-Only 2 genera form spores
+Bacillus
+Clostridium
2
Q
Sporulation
A
Bacteria exhaust all nutrients until they enter a developmental cycle that produces resting stages called spores/endospores
3
Q
Vegetative Cells
A
Live, active, reproducing cells
4
Q
Germination
A
Spore becomes a vegetative cell after nutrients become available.
5
Q
Bacteria we used for endospore
A
Bacillus subtilis
6
Q
Spores
A
- Resting stage
- Resistant to many things that would kill a vegetative cell
- Have a thick protein coat made of keratin called exosporium
7
Q
Endospore Stain
A
- Primary Stain: Malachite green (stains spores)
- Mordant: Steam from hot water bath
- Decolorizer: dH2O
- Counterstain: Safranin (stains vegetative cells)
