Microscopes Flashcards
What are the different parts of a microscope?
Eyepiece lens x10 Nose piece Objective lens x10,x20,x40 Stage Mirror course focus knob Fine focus knob
In terms of the objective lens, what magnifcation should you start with?
The lowest
What are the Limitations of light microscopes ?
Magnifcation compared to the high magnifcation of electron microscopes
What is resolution?
The ability of a microscope to distinguish detail
What is the main difference between light and electron microscopes?
Light has colour whilst electron is black and white
Light samples have to be alive whilst electron can be dead because it has to be in a vacuum
What is the equation for magnifcation?
Magnifcation= size of image divided by size of real object
What is magnification?
The amount an image is scaled up when viewed through a microscope
What is a positive of electron microscopes?
When viewing cells the organelles are observable
What is the equation for magnifcation?
Eyepiece lens x objective lens
Why is it eaiser to view cells from a low power first?
- Eaiser to focus
- Wider field of view
