Microscope Parts And Tests Flashcards
What is the eyepiece graticule?
A transparent piece of glass with a ruler etched on it added to the eyepiece of a microscope
What is the stage micrometer/ graticule?
Used to work out how much each division is worth on the eyepiece graticule
How do you calculate the distance of each eyepiece division?
Distance on stage micrometer (um) / number of eyepiece divisions
How can you use an eyepiece graticule and stage micrometer to measure the size of a structure?
•place micrometer on stage to calibrate eyepiece graticule
•line up scales on graticule and micrometer
•count how many graticule divisions are in the micrometer
•length of one eyepiece= 100um/number of divisions
•use calibrated values to calculate actual length