Different Types Of Microscopes

Question 1

How does a scanning electron microscope work?

Answer 1

•A beam of electrons is focused onto a specimens surface using electromagnetic lenses.
•Reflected electrons hit a collecting device and are amplified to produce an image on a photographic plate

Question 2

What’s the sem magnification and resolution?

Answer 2

M= x 100,000
R= 3-10nm

Question 3

How do transmission electron microscopes work?

Answer 3

Use a powerful beam of electrons to travel through a vacuum

Question 4

What are drawbacks to using electron microscopes?

Answer 4

•Expensive
•not suitable to view whole cells and tissues
•black and white images
•non living only
•large
•training required

Question 5

What’s the magnification and resolution of a tem?

Answer 5

M= x 500,000
R= 0.2 nm

Question 6

What is the magnification and resolution of a light microscope?

Answer 6

M= x1500
R= 200nm

Question 7

What do tem microscope produce?

Answer 7

An internal 2D image in black and white

Question 8

What do sem microscopes produce?

Answer 8

External 3D black and white image of the cell

Question 9

How does a confocal microscope work?

Answer 9

Uses a laser light to obtain image
Collects the image at different depths by scanning the laser through different focus positions
Allowing live specimens to be imaged without physical sectioning