microscope (C2)

Question 1

light microscope

Answer 1

• use visible light
• wavelength of 400-700nm
• max resolution 200nm
• max magnification 1500x

Question 2

phase contrast microscope

Answer 2

• special phase contrast condensers& objsctivelens

- enable living, non-pigmented specimen to be studied without fixing and staining

Question 3

transmission electron microscope

Answer 3

• resolving power of 1nm
• placed in vacuum to minimise electron scattering due to collision between electrons and molecules in the aie
• sections are treated with heavy metal (provides contrast)

Question 4

scanning electron microscope

Answer 4

• three-dimensional view
• dead pecimen coated with a thin layer of metal
• resolving power is 5nm higher than light microscope