Microscope

Question 1

Specimen preperation

Answer 1

Wet mount : drop of water to have specimen suspend between the slide and cover.
When putting a specimen, put smallest magnification adjust focus with fine adjustment knobs then go up a magnification & repeat until reaching desired magnification
Oil : focus at low power on specimen rotate partially to 100x and put oil drop on light circle then rotate fully to have 100x immersed in oil
Staining: methylen blue, gram stain, wright stain

Question 2

Setting up microscope

Answer 2

0. Adjust interpupillary distance and diopter adjustmenet ring (around the oculars)
1. Turn it on + 3-4V
2. Objective on smallest magnification: 4x or 10x
3. Check condenser diaphragm, should be almost closed
4. Put preparation in focus with the fine adjustment knobs. Top : vertical, Bottom: Horizontal

Question 3

Magnification

Answer 3

uni have 10x to 1000x
means smallest organism that can be seen is
0.180mm
Virus too small and worms too big

Question 4

Condenser

Answer 4

concentrate the light onto the specimen, its diaphragm regulates resolution, contrast and depth of field

Question 5

Low Power Objective

Answer 5

Small objective: 10x magnification

Low power scanning can be used to find best region of the smear : not too thin, not too thick (too much overlap)

Question 6

coarse adjustment knob

Answer 6

BIG knob located on the arm of the microscope moves the stage up and down to bring the specimen into focus