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Micropara Lab > Microscope > Flashcards

Microscope Flashcards

1
Q

Who created the a simple microscope in the 1500s

A

Anton Vaughn Leeuwonhoek

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2
Q

Coined term animalicule a.k.a microorganisms and is the father of microology or bacteriology

A

Anton Von Leeuwen Hoek

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3
Q

Who saw chamber like shapes on plant cells and called them cells?

A

Robert Hooke

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4
Q

What is microscopy?

A

Study of Microscopes

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5
Q

This is a type of microscope that allows light rays to pass directly to the eyes without being blocked by opaque plate in condenser?

A

Brightfeild Microscope

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6
Q

Components of a microscope

A
  • Framework
  • Stage
  • Light Source
  • Lens Systems
  • Focusing Knobs
  • Ocular Adjustments
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7
Q

basic frame structure of a microscope

A

arm and base

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8
Q

horizontal platform that supports the micro-
scope slide

A

stage

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9
Q

clamping device used for holding and moving the slide around on the stage

A

stage adjustment

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10
Q

it used to control the intensity of light

A

light intensity control

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11
Q

Most microscopes use this for reducing light intensity

A

neutral density filter

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12
Q

three lens systems of a microscope

A

oculars, the objectives, and the condenser

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13
Q

Part of the Microscope that has a diopter adjustment ring. Can be ocular or binocular.

A

Eye Peice

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14
Q

To make diopter adjustments, one focuses first with
the _______________ only.

A

right eye

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15
Q

this part collects and directs the light from the lamp to the slide being studied

A

condenser

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16
Q

regulates the amount of light that reaches the slide

A

diaphragm

17
Q

they are used for bringing objects into focus when studying an object on a slide

A

coarse adjustment and fine adjustment knob

18
Q

What objective lens should we always start with?

A

Scanner

19
Q

How do we find the total magnification?

A

(Magnification of Objective lens) x (Magnification of Ocular Lens) = Total Magnification

20
Q

For any light microscope, the limit of resolution
is about

A

0.2 μm

21
Q

What is the ideal magnification for the ocular lens?

A

10x

22
Q

laboratory microscopes have magnifications of 10x, 40x, and 100x. Name the three objective lens in the same order

A

low-power, high-dry, and oil immersion

23
Q

How do you transport a microscope?

A

Have one hand each on the arm and the base

24
Q

How do we take care of a microscope?

A
  • keep workstation clean
  • prevent any electric cords from dangling
  • use paper lens or liquids like ethyl alchohol to clean the lens and objective lens
  • place dust cover or in a cabinet with a lamp to prevent moisture to prevent fungus growth
25
Q

What are the characteristic of a good quality Brightfeild microscope?

A

Parfocal and Parcentral

26
Q

ability to completely separate two objects
in a microscopic field.

A

resolving power

27
Q

mathematical expression that describes how the condenser lens concentrates and focuses the light rays
from the light source

A

Numerical aperture

28
Q

range of distance in front of and behind a focused image within which other objects will appear clear and sharply defined

A

Depth of field

29
Q

it should be placed over the light source because the shorter wavelength of the
resulting light will provide maximum resolution. what is it called?

A

blue filter

30
Q
  • used between the slide
    and the 100 objective lens
  • special oil that has the same refractive index as glass.
A

Immersion oil

31
Q

powerful solvent that could
possibly dissolve the lens mounting cement in some
objective lenses if it were used too liberally

A

Acetone

32
Q

Various liquids can be used for cleaning microscope lenses like

A

Green soap with warm water, Xylene, Alcohol
and acetone

Micropara Lab (7 decks)

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