Microscope Flashcards
Who created the a simple microscope in the 1500s
Anton Vaughn Leeuwonhoek
Coined term animalicule a.k.a microorganisms and is the father of microology or bacteriology
Anton Von Leeuwen Hoek
Who saw chamber like shapes on plant cells and called them cells?
Robert Hooke
What is microscopy?
Study of Microscopes
This is a type of microscope that allows light rays to pass directly to the eyes without being blocked by opaque plate in condenser?
Brightfeild Microscope
Components of a microscope
- Framework
- Stage
- Light Source
- Lens Systems
- Focusing Knobs
- Ocular Adjustments
basic frame structure of a microscope
arm and base
horizontal platform that supports the micro-
scope slide
stage
clamping device used for holding and moving the slide around on the stage
stage adjustment
it used to control the intensity of light
light intensity control
Most microscopes use this for reducing light intensity
neutral density filter
three lens systems of a microscope
oculars, the objectives, and the condenser
Part of the Microscope that has a diopter adjustment ring. Can be ocular or binocular.
Eye Peice
To make diopter adjustments, one focuses first with
the _______________ only.
right eye
this part collects and directs the light from the lamp to the slide being studied
condenser
regulates the amount of light that reaches the slide
diaphragm
they are used for bringing objects into focus when studying an object on a slide
coarse adjustment and fine adjustment knob
What objective lens should we always start with?
Scanner
How do we find the total magnification?
(Magnification of Objective lens) x (Magnification of Ocular Lens) = Total Magnification
For any light microscope, the limit of resolution
is about
0.2 μm
What is the ideal magnification for the ocular lens?
10x
laboratory microscopes have magnifications of 10x, 40x, and 100x. Name the three objective lens in the same order
low-power, high-dry, and oil immersion
How do you transport a microscope?
Have one hand each on the arm and the base
How do we take care of a microscope?
- keep workstation clean
- prevent any electric cords from dangling
- use paper lens or liquids like ethyl alchohol to clean the lens and objective lens
- place dust cover or in a cabinet with a lamp to prevent moisture to prevent fungus growth
What are the characteristic of a good quality Brightfeild microscope?
Parfocal and Parcentral
ability to completely separate two objects
in a microscopic field.
resolving power
mathematical expression that describes how the condenser lens concentrates and focuses the light rays
from the light source
Numerical aperture
range of distance in front of and behind a focused image within which other objects will appear clear and sharply defined
Depth of field
it should be placed over the light source because the shorter wavelength of the
resulting light will provide maximum resolution. what is it called?
blue filter
- used between the slide
and the 100 objective lens - special oil that has the same refractive index as glass.
Immersion oil
powerful solvent that could
possibly dissolve the lens mounting cement in some
objective lenses if it were used too liberally
Acetone
Various liquids can be used for cleaning microscope lenses like
Green soap with warm water, Xylene, Alcohol
and acetone
