Culture Media Preperation Flashcards

1
Q

medium with the exact composition and amounts of the individual amino acids, vitamins, growth factors, and other components that make it up
are not exactly known

A

complex medium

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2
Q
  • Specific nutritional requirements for growth are known
  • specific chemical composition is known and individual components weighed out exactly to make up the medium
A

Defined Medium

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3
Q

Nutritional Requirements of Bacteria

A
  1. Carbon Source
  2. Energy Source
  3. Nitrogen
  4. Minerals
  5. Vitamins and Growth Factors
  6. Water
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4
Q

two groups based on their carbon requirements
- obtain their carbon from organic compounds
such as polysaccharides, carbohydrates, amino acids,
peptides, and protein

A

Heterotrophs

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5
Q

two groups based on their carbon requirements
- derive their carbon requirements from fixing carbon dioxide

A

autotrophs

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6
Q

Energy Source: Bacterial cells
- derive their energy needs from the breakdown
of organic molecules by fermentation or respiration

A

Chemoorganotrophs

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7
Q

Energy Source: Bacterial cells
- oxidize inorganic ions such as nitrate or iron to obtain energy to fix carbon dioxide

A

Chemolithotrophs

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8
Q

Energy Source: Bacterial cells
- contain photosynthetic
pigments such as chlorophyll or bacteriochlorophyll
that convert solar energy into chemical energy by
the process of photosynthesis

A

Photoautotrophs

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9
Q

Energy Source: Bacterial cells
- derive their energy requirements from photosynthesis, but their
carbon needs come from growth on organic molecules such as succinate or glutamate

A

photoheterotrophs

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10
Q

essential element in biolog-
ical molecules such as amino acids, nucleotide bases, and vitamins

A

Nitrogen

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11
Q

Example of nitrogen fixing bacteria

A

Rhizobium and Azotobacter

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12
Q
  • essential in bacterial metabo-lism because they are cofactors in enzymatic reactions and are integral parts of molecules such as cyto-chromes, bacteriochlorophyll, and vitamins
A

Minerals

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13
Q

serve as coenzymes in metabolism

A

Vitamins and Growth Factors

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14
Q

medium that allows
certain bacteria to grow but will inhibit others from
growing

A

selective medium

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15
Q

contains substances that
cause some bacteria to take on an appearance that
distinguishes them from other bacteria.

A

differential medium

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16
Q

original media used by Louis Pasteur

A

urine or meat broth

17
Q

Liquid medium is used for what?

A

diffuse growth

18
Q

Solid medium is used for what?

A

discrete colonies

19
Q

he cooked cut potato as early solid medium

A

Robert Koch

20
Q

he discovered agar from seaweeds and algae

A

Walther Hesse

21
Q

Classification of media based on consistency

A

Solid media
Semisolid media
Liquid media

22
Q

Classification of media based on consistency
- contains 2% agar
- Colony morphology, pigmentation, hemolysis can be appreciated

A

Solid media

23
Q

Classification of media based on consistency
- no agar
- For inoculum preparation, Blood culture, for the isolation of pathogens from a mixture.

A

Liquid media

24
Q

Classification of media based on consistency
- 0.5% agar

A

Semi solid medium

25
Q

Constituents Of Media

A
  • Agar or agar- agar
  • Peptone - mixture of partially digested proteins
  • Yeast or meat extract
  • NaCl
  • Melting point : 98°C
  • Solidifying point : 42°C
  • % of agar : Solid media (2%)
26
Q

Classification of media based on function or property
S.E.E.D. T.I.C.S

A
  • Simple media
  • Enriched media
  • Enrichment broth
  • Selective media
  • Indicator media
  • Differential media
  • Composite media
  • Transport media
27
Q

Classification of media based on function or property
- Also called Basal medium
- Most commonly used in routine labs

A

Simple Media

28
Q

Classification of media based on function or property
- Blood, serum or egg added to the basal medium.
- To grow bacteria which are more exacting in nutritional requirements

A

Enriched Media

29
Q
  • enriched medium often used to grow fastidious organisms and to differentiate bacteria based on their hemolytic properties
A

Blood Agar Media

30
Q

Classification of media based on function or property
- For mixed cultures or materials containing more than one bacterium
- Contains substances which stimulates wanted bacteria & inhibits unwanted
bacteria

A

Enrichment Broth (media)

31
Q

Classification of media based on function or property
- Substances added to a solid media to favour the growth of wanted bacteria

A

Selective Media

32
Q

Classification of media based on function or property
- used to culture non-fastidious microorganisms that do not need extra growth nutrients

A

composite media

33
Q

Classification of media based on function or property
– potato dextrose agar (PDA)
- Sabouraud Dextrose Agar (SDA)
– Fungi and yeast and also filamentous bacteria

A

Fungal Media

34
Q

Classification of media based on function or property
- Change in the colour of indicator with the growth of bacteria

A

Indicator Media

35
Q

Classification of media based on function or property
- To bring out differing characteristics of bacteria

A

Differential Media

36
Q

Classification of media based on function or property
- have buffer solutions containing carbohydrates, peptones and other nutrients (excluding growth factors) designed to preserve the viability of bacteria during transport without allowing them to multiply.

A

Transport Media

37
Q

MEDIA PREPARATION
CALCULATION

A

Using the formula: M1/V1 = M2/V2
– ratio and proportion

38
Q
A