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HISTOLOGY > Microscope > Flashcards

Microscope Flashcards

1
Q

Initial magnification happens in the____

A

objective lens

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2
Q

final magnification happens in the _____

A

ocular lens

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3
Q

limit of usable magnification

A

Resolving power

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4
Q

magnification of two dots that are no longer resolvable

A

Empty magnification

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5
Q

A measure of the number of highly diffracted image-forming light rays captured by the objective

A

Numerical aperture

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6
Q

The distance from the object being viewed to the lens or the objective

A

FOCAL LENGTH

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7
Q

foot on which the microscope rests

A

base

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8
Q

foot on which the microscope rests

A

arm

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9
Q

the platform in which the object being observed is placed

A

stage

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10
Q

tungsten or tungsten-halogen bulb, spectrophotometer

A

light source

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11
Q

controls the intensity of light

A

rheostat

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12
Q

directs and focuses light onto the material under examination

A

condenser

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13
Q

controls the amount of light that passes through

A

diaphragm

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14
Q

Already focused, just adjust the fine adjustment

A

parfocal

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15
Q

Alignment are centered

A

parcentric

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16
Q

to widen the distance of oculars

A

Interpupillary distance

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17
Q

to adjust the grade

A

Diopter adjustment

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18
Q

gives rapid movement over a wide range and is used to obtain an approximate focus

A

coarse

19
Q

gives very slow movement over a limited range and is used to obtain exact focus

A

fine

20
Q

Uses scattered light to view objects that appear light on a dark background
* Used to observe spirochetes: Treponema, Borrelia, Leptospira

A

darkfield microscope

21
Q

Gives objects a three-dimensional appearance * Useful for wet preparations (such as urine sediment), showing finer details without the need for special staining techniques * Add beam splitter (prism): will appear as 3D

A

DIFFERENTIAL INTERFERENCE-CONTRAST MICROSCOPE/NOMARSKY

22
Q

Provides greater magnification and resolution than conventional light microscopy * Black and white

A

ELECTRON MICROSCOPE

23
Q

Used to observe substances that fluoresce such as molecules coupled to antibodies for specific fluorescent labeling

A

FLUORESCENCE MICROSCOPE

24
Q

Used to increase the contrast in unstained specimens * Useful for counting platelets and observing cellular structures and casts in wet preparations of urine sediment and vaginal smears

A

PHASE-CONTRAST MICROSCOPE

25
Q
  • Used to identify crystals in body fluids: Synovial fluids found in joints
  • Useful clinically for differentiating between monosodium urate and calcium pyrophosphate dihydrate crystals in synovial fluid
A

Polarized and compensated polarized microscope

26
Q

Parallel yellow:
Parallel blue :

A

negative birefringence, positive birefringence

27
Q

Perpendicular yellow =
Perpendicular blue =

A

positive birefringence, negative birefringence

28
Q

study of tissues

A

histo

29
Q

study of disease

A

pathology

30
Q

Is the process of killing the tissue to preserve it * Police: to preserve, protect, and stabilize * Most commonly used fixative: 10% formalin

A

Fixation

31
Q

Removal of calcium or calcified tissues/tissue structures

A

Delcalcification

32
Q
  • Removal of water or moisture
  • Commonly used: Alcohols
  • Most preferred: 70-100% Ethanol
A

Dehydration

33
Q

One of the most common samples of clearing agent is Xylene or Chloroform; Tissue will appear as clear

A

Clearing

34
Q

to remove alcohol, xylene is being used to eradicate alcohol

A

Dealcoholization

35
Q

Adding wax such as paraffin and celloidin

A

infiltration/impregnation

36
Q

We are using embedding media to form the mold * Blocking/molding

A

Embedding

37
Q
  • Trim for microtome * Trimming of wax blocks
A

Trimming

38
Q

: used to trim tissue

A

Microtome

39
Q

Tissue ribboning

A

Sectioning/cutting

40
Q
  • Adding of color such as dyes to the tissues
  • To differentiate different structures of tissues
A

staining

41
Q

mounting

A

Putting it on the slides

42
Q
  • Secure the cover slide
  • To protect the tissue
A

ringing

43
Q
  • Naming/putting identity of the tissue * Always last yung paglabelling. * Putting identity of the tissue.
A

labelling

HISTOLOGY (9 decks)

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