Microscope Flashcards
a powerful tool that is commonly used in laboratories to observe biological structures and specimens that are too small to be seen with unaided eye.
Microscope
three major parts of microscope
- optical parts
- illuminating parts
- mechanical parts
two magnification lenses
the ocular lens
objective lens
usually magnify 10x
ocular lens
has three to four objective
lenses depending on the microscope used
objective lens
The four objectives are:
-scanner (magnifies 4x)
-low power objective (magnifies 10x),
-high power objective (magnifies 40x), –oil immersion objective
(magnifies 100x)
-a type of soft fine coarse paper that is used to clean and handle glass slides,
cover slips, and lenses without scratching or occurring damages to the glass wares.
-used to clean dirt, debris, and fingerprints
lens paper
-are glassware where the specimens are put.
-They are used in the
laboratory to keep the specimen in place.
-They function as a barrier and shield to the specimen from
winds, debris, or any external factors.
Glass slide and the cover slip
how to hold the microscope?
grab it by the neck and the bottom of the base
The microscope should
be placed on a workbench at least…..
five (5) inches from the edge
The microscope should be used in a way in which the ________ magnification lens is first
used up to the ________ magnification
-lowest
-highest
is the first used lens in microscopy
scanner lens
How to determine the total magnification?
multiply the magnification power
of the ocular lens and the objective lens used.
The diameter of the field observed under the microscope
field of view or field
of vision (FOV)
formula in measuring the size of FOV
(𝑭𝑶𝑽𝒍𝒐𝒘)(𝑴𝒂𝒈𝒍𝒐𝒘) = (𝑭𝑶𝑽𝒉𝒊𝒈𝒉)(𝑴𝒂𝒈𝒉𝒊𝒈𝒉)
the diameter of FOV of the low power objective
FOV low
the magnification of the low power objective
Maglow
the diameter of FOV of the high power objective
FOVhigh
the magnification of the high power objective
Maghigh
formula in measuring the circular area of FOV
𝑨𝒓𝒆𝒂 𝒐𝒇 𝑭𝑶𝑽 = (𝝅)(𝒓𝒂𝒅𝒊𝒖𝒔𝟐)
This tube holds the eyepiece in place.
Eyepiece
Tube
Classification of Eyepiece
Tube
Mechanical
Nosepiece holds the objective, often called a revolving turret.
You choose the objective lens by rotting to the specific lens
you want to use.
Nosepiece
Classification of Nosepiece
Mechanical
Scanning lens magnifies the specimen 4x. This lens is used
mainly to initially identify part of the specimen you want to
observe.
Scanning
Objective
Classification of Scanning
Objective
Optical
Low power objective magnifies the specimen 10x. This is used
to zoom in the specimen especially to samples that are difficult
to see without microscope.
Low Power
Objective
Classification of Low Power
Objective
Optical
High power objective magnifies the specimen 40x. This lens
is used to show specific details of the specimen the researcher
is observing.
High Power
Objective
Classification of High Power
Objective
Optical
the supports that holds the slides in place on
the stage.
Stage Clips
Classification of stage clips
Mechanical
controls the amount of light passing through
the slides.
Diaphragm
Classification of Diaphragm
Illuminating
project a parallel beam of light unto the sample
for illumination.
Light source
Classification of the light source
Illuminating
used to look through the microscope. It magnifies
the image of the specimen.
Eyepiece
Classification of the eyepiece
Optical
Nosepiece often called a ____________.
Revolving turret
connects the base to the nosepiece and eyepiece. It is
also used to carry the microscope.
Arm
Classification of the arm in microscope
Mechanical
This section in which the specimen is placed for drawing.
Stage
Classification of stage
Mechanical
moves the stage up and down to provide general
focus on the specimen.
Coarse
Focus
Classification of coarse focus
Mechanical
moves the stage in smaller increments to provide
a clear view of the specimen.
Fine Focus
Classification of fine focus
Mechanical
the main support of the microscope.
Base
Classification of base
Mechanical
Normal letter “e”
seen by the naked eye.
upside down letter “e”
seen under the microscope
A slide that is moved left while looking through the microscope, it will appear to move _________.
Right
the slide when moved down, it will seem to _________.
Move up
an ocular lens has ____ magnifying power.
10x
What does parfocal mean?
refers to objectives that can be changed with minimal or no refocusing.
when should the high objective power be used?
ideal for observing fine details within a specimen sample.
when should the low power objective be used?
cover a wide field of view and they are useful for examining large specimens or surveying many smaller specimens.
