Microscope Flashcards

(57 cards)

1
Q

a powerful tool that is commonly used in laboratories to observe biological structures and specimens that are too small to be seen with unaided eye.

A

Microscope

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2
Q

three major parts of microscope

A
  • optical parts
  • illuminating parts
  • mechanical parts
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3
Q

two magnification lenses

A

the ocular lens
objective lens

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4
Q

usually magnify 10x

A

ocular lens

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5
Q

has three to four objective
lenses depending on the microscope used

A

objective lens

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6
Q

The four objectives are:

A

-scanner (magnifies 4x)
-low power objective (magnifies 10x),
-high power objective (magnifies 40x), –oil immersion objective
(magnifies 100x)

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7
Q

-a type of soft fine coarse paper that is used to clean and handle glass slides,
cover slips, and lenses without scratching or occurring damages to the glass wares.
-used to clean dirt, debris, and fingerprints

A

lens paper

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8
Q

-are glassware where the specimens are put.
-They are used in the
laboratory to keep the specimen in place.
-They function as a barrier and shield to the specimen from
winds, debris, or any external factors.

A

Glass slide and the cover slip

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9
Q

how to hold the microscope?

A

grab it by the neck and the bottom of the base

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10
Q

The microscope should
be placed on a workbench at least…..

A

five (5) inches from the edge

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11
Q

The microscope should be used in a way in which the ________ magnification lens is first
used up to the ________ magnification

A

-lowest
-highest

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12
Q

is the first used lens in microscopy

A

scanner lens

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13
Q

How to determine the total magnification?

A

multiply the magnification power
of the ocular lens and the objective lens used.

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14
Q

The diameter of the field observed under the microscope

A

field of view or field
of vision (FOV)

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15
Q

formula in measuring the size of FOV

A

(π‘­π‘Άπ‘½π’π’π’˜)(π‘΄π’‚π’ˆπ’π’π’˜) = (π‘­π‘Άπ‘½π’‰π’Šπ’ˆπ’‰)(π‘΄π’‚π’ˆπ’‰π’Šπ’ˆπ’‰)

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16
Q

the diameter of FOV of the low power objective

A

FOV low

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17
Q

the magnification of the low power objective

A

Maglow

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18
Q

the diameter of FOV of the high power objective

A

FOVhigh

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19
Q

the magnification of the high power objective

A

Maghigh

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20
Q

formula in measuring the circular area of FOV

A

𝑨𝒓𝒆𝒂 𝒐𝒇 𝑭𝑢𝑽 = (𝝅)(π’“π’‚π’…π’Šπ’–π’”πŸ)

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21
Q

This tube holds the eyepiece in place.

A

Eyepiece
Tube

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22
Q

Classification of Eyepiece
Tube

A

Mechanical

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23
Q

Nosepiece holds the objective, often called a revolving turret.
You choose the objective lens by rotting to the specific lens
you want to use.

24
Q

Classification of Nosepiece

25
Scanning lens magnifies the specimen 4x. This lens is used mainly to initially identify part of the specimen you want to observe.
Scanning Objective
26
Classification of Scanning Objective
Optical
27
Low power objective magnifies the specimen 10x. This is used to zoom in the specimen especially to samples that are difficult to see without microscope.
Low Power Objective
28
Classification of Low Power Objective
Optical
29
High power objective magnifies the specimen 40x. This lens is used to show specific details of the specimen the researcher is observing.
High Power Objective
30
Classification of High Power Objective
Optical
31
the supports that holds the slides in place on the stage.
Stage Clips
32
Classification of stage clips
Mechanical
33
controls the amount of light passing through the slides.
Diaphragm
34
Classification of Diaphragm
Illuminating
35
project a parallel beam of light unto the sample for illumination.
Light source
36
Classification of the light source
Illuminating
37
used to look through the microscope. It magnifies the image of the specimen.
Eyepiece
38
Classification of the eyepiece
Optical
39
Nosepiece often called a ____________.
Revolving turret
40
connects the base to the nosepiece and eyepiece. It is also used to carry the microscope.
Arm
41
Classification of the arm in microscope
Mechanical
42
This section in which the specimen is placed for drawing.
Stage
43
Classification of stage
Mechanical
44
moves the stage up and down to provide general focus on the specimen.
Coarse Focus
45
Classification of coarse focus
Mechanical
46
moves the stage in smaller increments to provide a clear view of the specimen.
Fine Focus
47
Classification of fine focus
Mechanical
48
the main support of the microscope.
Base
49
Classification of base
Mechanical
50
Normal letter "e"
seen by the naked eye.
51
upside down letter "e"
seen under the microscope
52
A slide that is moved left while looking through the microscope, it will appear to move _________.
Right
53
the slide when moved down, it will seem to _________.
Move up
54
an ocular lens has ____ magnifying power.
10x
55
What does parfocal mean?
refers to objectives that can be changed with minimal or no refocusing.
56
when should the high objective power be used?
ideal for observing fine details within a specimen sample.
57
when should the low power objective be used?
cover a wide field of view and they are useful for examining large specimens or surveying many smaller specimens.