Microscope Flashcards

1
Q

a powerful tool that is commonly used in laboratories to observe biological structures and specimens that are too small to be seen with unaided eye.

A

Microscope

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2
Q

three major parts of microscope

A
  • optical parts
  • illuminating parts
  • mechanical parts
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3
Q

two magnification lenses

A

the ocular lens
objective lens

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4
Q

usually magnify 10x

A

ocular lens

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5
Q

has three to four objective
lenses depending on the microscope used

A

objective lens

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6
Q

The four objectives are:

A

-scanner (magnifies 4x)
-low power objective (magnifies 10x),
-high power objective (magnifies 40x), –oil immersion objective
(magnifies 100x)

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7
Q

-a type of soft fine coarse paper that is used to clean and handle glass slides,
cover slips, and lenses without scratching or occurring damages to the glass wares.
-used to clean dirt, debris, and fingerprints

A

lens paper

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8
Q

-are glassware where the specimens are put.
-They are used in the
laboratory to keep the specimen in place.
-They function as a barrier and shield to the specimen from
winds, debris, or any external factors.

A

Glass slide and the cover slip

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9
Q

how to hold the microscope?

A

grab it by the neck and the bottom of the base

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10
Q

The microscope should
be placed on a workbench at least…..

A

five (5) inches from the edge

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11
Q

The microscope should be used in a way in which the ________ magnification lens is first
used up to the ________ magnification

A

-lowest
-highest

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12
Q

is the first used lens in microscopy

A

scanner lens

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13
Q

How to determine the total magnification?

A

multiply the magnification power
of the ocular lens and the objective lens used.

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14
Q

The diameter of the field observed under the microscope

A

field of view or field
of vision (FOV)

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15
Q

formula in measuring the size of FOV

A

(𝑭𝑶𝑽𝒍𝒐𝒘)(𝑴𝒂𝒈𝒍𝒐𝒘) = (𝑭𝑶𝑽𝒉𝒊𝒈𝒉)(𝑴𝒂𝒈𝒉𝒊𝒈𝒉)

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16
Q

the diameter of FOV of the low power objective

A

FOV low

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17
Q

the magnification of the low power objective

A

Maglow

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18
Q

the diameter of FOV of the high power objective

A

FOVhigh

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19
Q

the magnification of the high power objective

A

Maghigh

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20
Q

formula in measuring the circular area of FOV

A

𝑨𝒓𝒆𝒂 𝒐𝒇 𝑭𝑶𝑽 = (𝝅)(𝒓𝒂𝒅𝒊𝒖𝒔𝟐)

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21
Q

This tube holds the eyepiece in place.

A

Eyepiece
Tube

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22
Q

Classification of Eyepiece
Tube

A

Mechanical

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23
Q

Nosepiece holds the objective, often called a revolving turret.
You choose the objective lens by rotting to the specific lens
you want to use.

A

Nosepiece

24
Q

Classification of Nosepiece

A

Mechanical

25
Q

Scanning lens magnifies the specimen 4x. This lens is used
mainly to initially identify part of the specimen you want to
observe.

A

Scanning
Objective

26
Q

Classification of Scanning
Objective

A

Optical

27
Q

Low power objective magnifies the specimen 10x. This is used
to zoom in the specimen especially to samples that are difficult
to see without microscope.

A

Low Power
Objective

28
Q

Classification of Low Power
Objective

A

Optical

29
Q

High power objective magnifies the specimen 40x. This lens
is used to show specific details of the specimen the researcher
is observing.

A

High Power
Objective

30
Q

Classification of High Power
Objective

A

Optical

31
Q

the supports that holds the slides in place on
the stage.

A

Stage Clips

32
Q

Classification of stage clips

A

Mechanical

33
Q

controls the amount of light passing through
the slides.

A

Diaphragm

34
Q

Classification of Diaphragm

A

Illuminating

35
Q

project a parallel beam of light unto the sample
for illumination.

A

Light source

36
Q

Classification of the light source

A

Illuminating

37
Q

used to look through the microscope. It magnifies
the image of the specimen.

A

Eyepiece

38
Q

Classification of the eyepiece

A

Optical

39
Q

Nosepiece often called a ____________.

A

Revolving turret

40
Q

connects the base to the nosepiece and eyepiece. It is
also used to carry the microscope.

A

Arm

41
Q

Classification of the arm in microscope

A

Mechanical

42
Q

This section in which the specimen is placed for drawing.

A

Stage

43
Q

Classification of stage

A

Mechanical

44
Q

moves the stage up and down to provide general
focus on the specimen.

A

Coarse
Focus

45
Q

Classification of coarse focus

A

Mechanical

46
Q

moves the stage in smaller increments to provide
a clear view of the specimen.

A

Fine Focus

47
Q

Classification of fine focus

A

Mechanical

48
Q

the main support of the microscope.

A

Base

49
Q

Classification of base

A

Mechanical

50
Q

Normal letter “e”

A

seen by the naked eye.

51
Q

upside down letter “e”

A

seen under the microscope

52
Q

A slide that is moved left while looking through the microscope, it will appear to move _________.

A

Right

53
Q

the slide when moved down, it will seem to _________.

A

Move up

54
Q

an ocular lens has ____ magnifying power.

A

10x

55
Q

What does parfocal mean?

A

refers to objectives that can be changed with minimal or no refocusing.

56
Q

when should the high objective power be used?

A

ideal for observing fine details within a specimen sample.

57
Q

when should the low power objective be used?

A

cover a wide field of view and they are useful for examining large specimens or surveying many smaller specimens.