Microscope Flashcards
a powerful tool that is commonly used in laboratories to observe biological structures and specimens that are too small to be seen with unaided eye.
Microscope
three major parts of microscope
- optical parts
- illuminating parts
- mechanical parts
two magnification lenses
the ocular lens
objective lens
usually magnify 10x
ocular lens
has three to four objective
lenses depending on the microscope used
objective lens
The four objectives are:
-scanner (magnifies 4x)
-low power objective (magnifies 10x),
-high power objective (magnifies 40x), –oil immersion objective
(magnifies 100x)
-a type of soft fine coarse paper that is used to clean and handle glass slides,
cover slips, and lenses without scratching or occurring damages to the glass wares.
-used to clean dirt, debris, and fingerprints
lens paper
-are glassware where the specimens are put.
-They are used in the
laboratory to keep the specimen in place.
-They function as a barrier and shield to the specimen from
winds, debris, or any external factors.
Glass slide and the cover slip
how to hold the microscope?
grab it by the neck and the bottom of the base
The microscope should
be placed on a workbench at least…..
five (5) inches from the edge
The microscope should be used in a way in which the ________ magnification lens is first
used up to the ________ magnification
-lowest
-highest
is the first used lens in microscopy
scanner lens
How to determine the total magnification?
multiply the magnification power
of the ocular lens and the objective lens used.
The diameter of the field observed under the microscope
field of view or field
of vision (FOV)
formula in measuring the size of FOV
(𝑭𝑶𝑽𝒍𝒐𝒘)(𝑴𝒂𝒈𝒍𝒐𝒘) = (𝑭𝑶𝑽𝒉𝒊𝒈𝒉)(𝑴𝒂𝒈𝒉𝒊𝒈𝒉)
the diameter of FOV of the low power objective
FOV low
the magnification of the low power objective
Maglow
the diameter of FOV of the high power objective
FOVhigh
the magnification of the high power objective
Maghigh
formula in measuring the circular area of FOV
𝑨𝒓𝒆𝒂 𝒐𝒇 𝑭𝑶𝑽 = (𝝅)(𝒓𝒂𝒅𝒊𝒖𝒔𝟐)
This tube holds the eyepiece in place.
Eyepiece
Tube
Classification of Eyepiece
Tube
Mechanical