Microorganisms Flashcards
single cell complete organism that can grow, metabolize, reproduce
bacteria
bacterial cell walls are made up of
mucopolysaccharide
most common response to a bacterial infection that is stained with H&E
neutrophils
H&E shows these 3 things
necrosis, abscesses and granulomas
the gram is usually ordered 1st for this organism
bacterfia
used to determine whether an abscess or necrosis is bacterial in origin
gram stains
3 infections that can be clarified with a gram stain
meningitis, pneumonia, wounds
allows classification of bacilli into acid-fast and non acid-fast bacilli
acid fast stains
important test for mycobacterium bacilli containing thick waxy cell walls
acid fast stains
organism of tuberculosis
M. tuberculosis
agent of leprosy
M. leprae
acid fast bacterial cell wall stain this color
bright red
initial stain of acid fast stain
carbol fuchsin
counter stain of acid fast stain
methylene blue
other bacterial background tissue and components stain this color after decolorization and counter stain
blue
fluorescent stain that stains TB, utilizing heat
auramine-rhodamine fluorescent technique
mycobacteria appear this color against a greenish background in A-R fluorescent stain
bright yellow or orange
mycobacteria show this color fluorescence when examined under a fluorescent microscope
red-yellow
disease produced by fungus
mycosis
fungal walls are rich in
polysaccharides
can detect fungi in sputum, lung biopsies, skin infections
GMS stain
when polysaccharides are oxidized they form reactive aldehyde groups which can be detected by these types of stains
silver
tissue is oxidized then impregnated with methenamine silver solution, then counterstained to visualize
fungus
agryophillic so absorb silver from solution, but need reducing solution to make silver visible
spirochetes
used to demonstrate syphilis, H. pylori, cat scratch fever
warthin-starry stain
Dieterle’s, Steiner are stains that stain
spirochetes
curved or spiral shaped bacteria
H. pylori
found in high percentages in the stomach/duodenum of patients with chronic gastritis or duodenal ulcers
h. pylori
associated with duodenal and gastric ulcers, and gastric malignancies
gastritis due to h. pylori
can weaken protective coating of stomach and duodenum, allowing digestive juice to irritate linings
h. pylori
if there is a clinical history of chronic gastritis, order this stain
H pylori
