Connective Tissue Stains

Question 1

extracellular insoluble protein with rubbery like consistency

Answer 1

elastin

Question 2

stains poorly with H&E

Answer 2

elastin

Question 3

gives elasticity to tissue and organs, abundant in tissues requiring flexibility

Answer 3

elastic fibers

Question 4

allows recovery of tissue shape after normal deformation or stretching

Answer 4

elastic fibers

Question 5

prominent in lungs, bladder, arterial walls and skin

Answer 5

elastin

Question 6

help identify tumor invasion of large blood vessels

Answer 6

elastic stains

Question 7

helps determine in lung specimens if the tumor is invading the pleura

Answer 7

elastin stains

Question 8

useful in identifying AVMs, aneurysms, emphysema, vasculitis

Answer 8

elastic stains

Question 9

mixture of alcoholic hematoxylin, ferric chloride, iodine

Answer 9

Verhoeff van Gieson

Question 10

overstaining the tissue then differentiating with ferric chloride is the most important step in this stain

Answer 10

Verhoeff van Gieson

Question 11

elastic fibers are stained this color in a VvG stain

Answer 11

black

Question 12

nuclei are stained this color in a VvG stain

Answer 12

black-blue

Question 13

collagen is stained this color in a VvG stain

Answer 13

red

Question 14

other tissue elements are stained this color in a VvG stain

Answer 14

yellow

Question 15

assess the degree of fibrosis in the liver

Answer 15

medical liver biopsies

Question 16

bronchovascular structure in lung is stained by

Answer 16

trichome

Question 17

capsules around organs are stained by

Answer 17

trichome

Question 18

distinguishing muscle from collagen (indicating fibrotic change) is stained by

Answer 18

trichome

Question 19

pyelonephritis is stained by

Answer 19

trichome

Question 20

neuromuscular disease histological changes are stained by

Answer 20

trichome

Question 21

distinguishing tumors rising from muscle cells vs fibroblasts is stained by

Answer 21

trichome

Question 22

3 colors to distinguish between collagen, muscle and fibrin

Answer 22

Gomori/Masson trichome stain

Question 23

nuclei are stained this color in GMT stain

Answer 23

black

Question 24

muscle fibers and cytoplasm are stained this color in GMT stain

Answer 24

red

Question 25

collagen and mucin are stained this color in GMT stain

Answer 25

blue-green

Question 26

agryophillic fiber (attracted to silver)

Answer 26

reticulin fiber

Question 27

most abundant in the liver, spleen and kidney

Answer 27

reticulin fiber

Question 28

useful in demonstrating disease state in organs by demonstrating reticular patterns not normal

Answer 28

reticulin stains

Question 29

useful for demonstrating liver architecture, hepatocyte necrosis and regeneration

Answer 29

reticulin stain

Question 30

reticulin surrounds nests of tumor cells in this pathology

Answer 30

carcinoma

Question 31

reticulin is mesh like and surrounds individual cells in this pathology

Answer 31

sarcoma

Question 32

metallic compound such as ammonium sulfate is deposited around the reticulin fibers

Answer 32

sensitization

Question 33

ammonical silver solution is reduced by the exposed aldehyde groups to metallic silver

Answer 33

silver impregnation

Question 34

exposing the tissue to formaldehyde to make it visible

Answer 34

developing

Question 35

bound silver is treated with gold chloride, turning brown to black

Answer 35

toning

Question 36

hexose sugar in reticulin fibers yield aldehydes

Answer 36

oxidation

Question 37

most commonly caused by alcoholic liver disease, nonalcoholic steatosis or hepatitis

Answer 37

cirrhosis

Question 38

nuclei in GMT stain is stained by

Answer 38

iron hematoxylin

Question 39

cytoplasm, muscle and collagen in a GMT stain is stained by

Answer 39

Biebrich scarlet

Question 40

Biebrich scarlet is diffused out of cytoplasm by this in a GMT stain

Answer 40

phosphotungstic acid

Question 41

collagen picks up this stain in a GMT stain

Answer 41

aniline blue