Microbiology: PCR practical

Question 1

What sort of gel is used in PCR?

Answer 1

Agarose

Question 2

Which enzyme is used in the reaction mixture for PCR?

Answer 2

Taq polymerase

Question 3

Recall the name and temperature of each cycle of PCR

Answer 3

Denaturation: 94
Annealing: 54
Extension: 72

Question 4

Recall the direction of movement of DNA in electrophoresis

Answer 4

Negative –> positive (due to negative phosphate backbone)

Question 5

Recall 3 uses of PCR

Answer 5

Tissue typing for transplants
HIV detection
Genetic testing

Question 6

Recall 3 advantages of PCR

Answer 6

Simple, fast, sensitive

Question 7

Recall 2 cons of PCR

Answer 7

Need prior knowledge of target sequence, small amounts of contaminated fragment will be amplified