Microbiology Lab Quiz 2 Flashcards
aerobic respiration
most ATP produced
fermentation
least ATP produced
anaerobic respiration
nitrate reduction
why does E. coli turn pink after the gram stain procedure?
the thin peptidoglycan layer
the gram stain: list the reagents in order
crystal violet, iodine, ethyl alcohol, safranin
which is an acid-fast bacteria?
Mycobacterium
acid fast: what is the primary stain and mordant of this procedure?
primary stain: carbol fuchsin, mordant: time
what does the yellow color in the glucose tubes represent?
growth
I have 3 tubes of glucose broth, they are incubated in 15, 37, and 55 degrees C. If the bacteria grew in the 15 degree tube what would it be classified as? 37? 55?
15: psychrophiles, 37: mesophiles, 55: thermophiles
in what solution type does plasmolysis occur?
hypertonic
how do most bacteria reproduce?
binary fission
will a sensitive or resistant bacteria grow on NAS?
resistant
what non-ionizing radiation did we use in class?
UV
which two lights are used to control growth?
UV and infared
does photoreactivation or photooxidation damage the cell?
photooxidation
what happens to MSA when Staph aureus grows on it?
S. aureus can ferment on mannitol, red -> yellow media
which blood hemolysis type completely lyses the cell and has a strong yellow background?
beta hemolysis
what is 25 percent NaCl? .85 percent?
25: plasmolysis .85: isotonic
NA is:___ NAS is:___ growth on the NAS is:___
sensitive, resistant, resistant
obligate aerobe growth
top, M. luteus
obligate anaerobe growth
bottom, C. sporogenes
facultative anaerobe growth
throughout, E. coli
ionizing radiation and examples
high energy short wavelengths, gamma and xray
nonionizing radiation
low energy long wavelengths, ultraviolet and infrared
