Microbiology Lab Quiz 2

Question 1

aerobic respiration

Answer 1

most ATP produced

Question 2

fermentation

Answer 2

least ATP produced

Question 3

anaerobic respiration

Answer 3

nitrate reduction

Question 4

why does E. coli turn pink after the gram stain procedure?

Answer 4

the thin peptidoglycan layer

Question 5

the gram stain: list the reagents in order

Answer 5

crystal violet, iodine, ethyl alcohol, safranin

Question 6

which is an acid-fast bacteria?

Answer 6

Mycobacterium

Question 7

acid fast: what is the primary stain and mordant of this procedure?

Answer 7

primary stain: carbol fuchsin, mordant: time

Question 8

what does the yellow color in the glucose tubes represent?

Answer 8

growth

Question 9

I have 3 tubes of glucose broth, they are incubated in 15, 37, and 55 degrees C. If the bacteria grew in the 15 degree tube what would it be classified as? 37? 55?

Answer 9

15: psychrophiles, 37: mesophiles, 55: thermophiles

Question 10

in what solution type does plasmolysis occur?

Answer 10

hypertonic

Question 11

how do most bacteria reproduce?

Answer 11

binary fission

Question 12

will a sensitive or resistant bacteria grow on NAS?

Answer 12

resistant

Question 13

what non-ionizing radiation did we use in class?

Answer 13

UV

Question 14

which two lights are used to control growth?

Answer 14

UV and infared

Question 15

does photoreactivation or photooxidation damage the cell?

Answer 15

photooxidation

Question 16

what happens to MSA when Staph aureus grows on it?

Answer 16

S. aureus can ferment on mannitol, red -> yellow media

Question 17

which blood hemolysis type completely lyses the cell and has a strong yellow background?

Answer 17

beta hemolysis

Question 18

what is 25 percent NaCl? .85 percent?

Answer 18

25: plasmolysis .85: isotonic

Question 19

NA is:___ NAS is:___ growth on the NAS is:___

Answer 19

sensitive, resistant, resistant

Question 20

obligate aerobe growth

Answer 20

top, M. luteus

Question 21

obligate anaerobe growth

Answer 21

bottom, C. sporogenes

Question 22

facultative anaerobe growth

Answer 22

throughout, E. coli

Question 23

ionizing radiation and examples

Answer 23

high energy short wavelengths, gamma and xray

Question 24

nonionizing radiation

Answer 24

low energy long wavelengths, ultraviolet and infrared

Question 25

blood agar

Answer 25

differential media

Question 26

MSA

Answer 26

selective and differential media

Question 27

beta hemolysis

Answer 27

complete hemolysis

Question 28

alpha hemolysis

Answer 28

partial hemolysis

Question 29

gamma hemolysis

Answer 29

no hemolysis