microbiology lab 2 Flashcards

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1
Q

how can you describe a colony?

A

punctiform, circular, rhizoid, irregular, filamentous

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2
Q

how can you describe the edge?

A

entire, undulate, lobate, filamentous, curled

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3
Q

how can you describe the surface?

A

smooth and glistening, rough, wrinkled, dry or powdery

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4
Q

how can you describe the elevation?

A

flat, raised, covex, pulvinate, umbonate

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5
Q

what is enteric?

A

bacteria that exist in the intestines of animals/humans

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6
Q

what is aerobic?

A

bacteria that can grow/live when oxygen is present

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7
Q

what is anaerobic?

A

bacteria that do not require oxygen for growth and cannot have present

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8
Q

what is pathogenic?

A

a microbe able to cause damage in susceptible host

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9
Q

what does it mean when colonies on BAP present with red surrounding colonies?

A

non-hemolytic bacteria present (gamma hemolysis)

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10
Q

what does it mean if colonies are present and surrounded by a green zone?

A

partially hemolytic bacteria present (alpha hemolysis)

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11
Q

what does it mean if colonies are present and surrounded by a transparent clear zone?

A

completely hemolytic bacteria present (beta hemolysis)

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12
Q

what does it mean if no colonies are present?

A

no bacteria present in sampel or accidentally killed in incubation of plate by overheating

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13
Q

what will the make plate grow?

A

designed to grow gram negative bacteria

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14
Q

what does it mean if no bacteria are present on MAC plate?

A

cannot grow in prescene of bile salts/ crystal violet
gm+ and non-enteric

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15
Q

what does it mean if colonies are visable and are white?

A

gm- enteric bacteria are lac- therefore, cannot use lactose and instead metabolize the peptide in the media,

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16
Q

what does it mean if colonies are visible and pink?

A

gm- enteric bacteria are lac+ and use lactose, produce acid, and activate neutral red dye

17
Q

what is purpose of flaming loop?

A

ensures bacteria are not transferred to bench

18
Q

what is purpose of heat fixing?

A

kills the bacteria and causes bacteria cells to adhere to slide

19
Q

what is prupose of crystal violot?

A

stains peptidoglycan in bacteria cell wall, especially in thick layer (gm+)

20
Q

what is purpose of grams iodine?

A

application of mordant, forms insoluable complex with crystal violet fixing the stain in cell wall

21
Q

what is purpose of acetone- alcohol?

A

gm+ dehydrates cell, trapping crystal violet and iodine, (gm-) dissolves lipopolysaccride in outer membrane and washes stain excess

22
Q

what is safranin?

A

stains thinner peptidoglycan coat of gram- cells pink, so can see these cells lost their stain

23
Q

what causes nasocomial infections?

A

poor hand hygeine
contaminated fomites
surgeries (poor aspesis)

24
Q

what are impacts of nasocomial infections?

A

loss of rom or mobility
250,000 deaths
longer stay in hosptial

25
Q

prevention of nasocomial infections? a

A

aspesis, hand hygiene, disinfecting surfaces

26
Q

what is MRSA?

A

antiobiotic resistant staph infection

27
Q

what is VRE?

A

cause of antibiotic resistant gastrointestinal infection