Microbiology Exam 1 Flashcards
Why is microbiology important for veterinary curriculum?
Gain knowledge in infectious disease of animals | Diagnose-> Treat -> Prevent
In Bloom's Taxonomy of Learning, A. Cognitive= doing/ hands B. Cognitive= knowing/ head C. Affective = knowing/ head D. Affective = feeling/ heart E. Psychomotor= doing/ hands F. A, C, E G. B, D, E
G. B, D, E
Microbiology: A. Study of people B. Study of small life C. Study of microscopes D. Study of animals
B. Study of Small Life
T/F: Microbes are beneficial and harmful
True: Harmful in disease and food spoilage
beneficial with probiotics and fermentation, antibiotics, vaccines, vitamins, etc..
What is the OneHealth Model?
A. Humans, Animals, Environment
B. Humans, Gas, Oil
C. Social, Mental, Physical
A. Humans, Animals, Environment
Who made the first compound microscope ? A. Hooke B. Aristotle C. Janssen D. Pasteur
C. Janssen
T/F: Hooke had the first public depiction of a microorganism
True
Van Leeuwanhoek showed protozoa and bacteria; is this true?
Yes
Scanning Electron Microscopes run light thru internal image whereas Transmission show a 3D surface image: T/F
FALSE: It is the opposite
All of these were contributions from Pasteur except: A. Disproving spontaneous generation B. Proposed Germ Theory C. Vaccine for rabies and anthrax D. First depiction of a microorganism E. Pasterization
D. First depiction of a microorganism was Hooke
These are all koch’s postulates except for one:
- microorganisms must be found in all suffering not healthy
- microorganisms must be isolated from diseased and grown in pure culture
- Cultured microorganisms should cause disease when introduced to healthy
- microorganisms must be re isolated from inoculated diseased experimental host and identified as being identical to the original specific causative agent
- red bull gives you wings
5
All these people were involved in spontaneous generation except: A. Pasteur B. Hooke C. Aristotle D. Redi E. Needham & Spallanzani
B. Hooke
The study of evolutionary history of living organisms is: A. Phylogeny/ Systematics B. Taxonomy C. History D. Biology
A. Phylogeny/ Systematics
T/F: David Bruce is associated with trypanosomes
True
Who is the father of antiseptic surgery?
Joe Lister
What is Jenner known for?
Smallpox
Who is the father of modern pathology (zoonosis)?
Rudolf Virchow
Who discovered penicillin?
Flemming
Who discovered phagocytosis?
Metchnikoff
All biological macromolecules are made of all of the following except: A. Carbon B. Hydrogen C. Oxygen D. Nitrogen E. Sugar F. Sulfar G. Phosphorous
E. Sugar
T/F: All living things have a plasma membrane, use ATP, genetic info in DNA
TRUE
Population that can breed and produce fertile offspring: A. group B. Species C. Crowd D. Genotype
B. Species
What is taxonomy?
study of classification of living objects: Linnaeus
The modern classification of living objects is called:
Binomial Nomenclature
What are the current 3 domain classifications?
A. Prokaryotes, Eukaryotes, Archea
B. Bacteria, Archea, Eukaryota
C. Eubacteria, Eukarya, Prokarya
B. Bacteria, Archea, Eukaryota
T/F: Eukaryotes have no nuclear membrane, no membrane enclosed organelles, no carbohydrates, no sterols, no cytoskeleton, a ribosome 70s, binary fission, no meiosis
False: Those are the characteristics of prokaryotes; eukaryotes have a true nucleus, have lysosomes, golgi, ER, mitochondria, and chloroplasts, have sterols and carbohydrates, a cytoskeleton, ribosome 80s and 70s, use mitosis, involves meiosis
T/F: Archea are prokaryotic and lack peptidoglycan
True: they do not undergo binary fission, they are found in extreme environments: like halophiles and thermophiles
Eukaryota include: A. protozoans B. fungi C. algae D. Animals and plants E. All of the Above
E. All of the above
T/F: a virus is a small infectious agent that replicates inside living cells, has genetic material, a protein coat, and an envelope
True
How do you identify bacteria?
Culture analysis of morphological characteristics and differential staining, biochemical tests, serology, phage typing, fatty acid profiling, nucleic acid base testing , mass spectrometry, PCR
How do you classify parasites?
Protazoa, 1 cell, amoeba metazoan, multi cell is helminths
How do we learn about relationships between living objects? A. Fossil Records B. Comparative Homologies C. Comparative Sequences D. All of the above
D. All of the above
Molecular Biology…
A. study of molecular foundation of the process of replication, transcription, translation, and cell function
B. study of small living organisms
C. Study of life
A
DNA, RNA, Protein and their biosynthesis, regulation, and interactions: Their genetic material must be able to all the following except:
A. contain info necessary to make whole organism
B. pass from parent to offspring and from cell to cell during cell division
C. be accurately copied
D. account for the known variation within and between species
E. Generate new genes from dead genes
E.
Who were the people to reveal DNA is the genetic material in transformation? A. Avery B. MacLeod C. McCarty D. Watson E. A, B, C
E. A, B, C
Who did bacterial transformation?
Griffith with the rough and smooth strains that had capsules
Who came up with the DNA structure of the Double Helix?
Watson and Crick using Paulings method, with assistance from Franklin
Chargaffs rules state that:
A. A-T C-G
B. A-T = C-G
C. G-T = A-C
A. A to T C to G
Nucleic acids have what three basic components? A. Phosphate B. Sugar C. Nitrogenous Base D. All of the above
D. All of the above
T/F: RNA stores and transmits genetic info
False: DNA does that; RNA translates info coded in DNA for protein production
T/F: Nucleotides hace a phosphate group, hexose, and nitrogenous base
False: They have a pentose sugar not hexose
T/F: a phosphodiester bond links 2 sugars
True
Which is not true about DNA: A. double stranded helical B. Sugar Phosphate C. Bases on outside D. Stable by H-Bonds E. Base pairing with char gaffs rules
C. Bases on outside
They are actually on the inside
DNAs 3 components include:
A. Phosphate Group, Deoxyribose, Nitrogenous base (C, G, A, T)
B. Phosphate Group, Ribose, Nitrogenous Base (C, G, A, U)
A
T/F: DNA has 10 bases per turn, 2 strands that are complimentary and antiparallel
True
T/F: Replication happens in minor groove
False: Major groove
The process involved in replication matches which statement?
A. 2 strands of parental separate, template, new nucleotides obey chargaffs rules, end result is 2 new double helix with same base sequence as parent.
B. 2 strands of parental separate, one is template, and other disassociates, nucleotides pair up randomly and eat end result is 1 new double helix
A is the correct statment
The ori (origin of replication)... A. Fork B. Fragments C. start point of replication D. provides rep bubble that forms E. both C and D
E. Both C and D
T/F: Synthesis begins with a primer, proceeds in a 5’–3’ direction, leading strand made synthesis continues forward, lagging strand is made in okazaki fragments and connects later
True
T/F: DNA helices binds to DNA 5’–3’ separating while moving fork forward
True