Microbiology Chap 3

Question 0

Microscope terms? Resolution, magnification, refractive index amd use of immersion oil

Answer 0

Res=the ability of the lenses to distingiish two points
Total magnification= objective lens c ocular lens
Ref index= light bending ability of medium
Keep light from bending too much

Question 1

What does compound lught, darfield/brightfield, phase contrast, differential interference contrast, floresence and confocal have on common?

Answer 1

All lighy Microscopies that uses visible light

Question 2

Brightfield vs darkfield and what anout phase contrast microscopy

Answer 2

Dark objects visible against bright back ground capitalize on different light refraction

Light objects against dark background
Only light reflected from organism enters lens

Uses collection of lights, do not need fix specimen in pahse = light out phase = dark

Question 3

Differential interference contrast microscopy

Answer 3

Accentuates diffraction through specimen and prism of colors with two light beams used

Question 4

Flourescence microscopy

Answer 4

Uses uv light,glouresbxe dyes used for immunoflourescences

Question 5

Confocal microscopy

Answer 5

Florochrome dyes
Short wavelength to excite
Produce 3d image
Computers

Question 6

Two photon microscopy

Answer 6

Fluorochrome fyes, two lobg photon wavelength

Used to see cells attached to a surface 1mm deep

Question 7

Scanning acoustic micracopy

Answer 7

Measures sound waves resolution 1um

Question 8

Electron micropies?between sam and tem

Answer 8

Tem= transmission electron mic
Ultra thin specimens stained with haevy metal salts

Sam=scanning electron mic
Electron gun scan surface observe secondary electrons emitted fron specimen

Question 9

Scanned probe microscopy differences? Stm and afm

Answer 9

Scanning tunneling microscopy? Uses metal probe

Atomoic force microscopy= metal and diamond probe and 3d

Question 10

define smear and stain, and explain components of stains and negative staining

Answer 10

Smear=thinfilm of solution microbes in a slide
Staining=coloring the microbe with a dye

Components? Basic dye has cation and acid dyes have anion

Negative stain is stainibg the background

Question 11

How simple stainworks

Answer 11

Single dye & mordant to hold stai or cpat specimen

Question 12

Differences between the two differential stains

Answer 12

Gram stain classify gram positive(purple) amd negative(red) bacteria
Promary stain:CV decolorizer:alchol acetone counterstain:safrinin

P

Question 13

Special stains?

Answer 13

Capsule, : negative stainex

endsospore : primary malachtie green amd heat , decolor: water, cpunter stain safrinin

and flagella : mordant and carbofucshinsimple stain

Question 14

Acid fast stains? Mecahins and resultp

Answer 14

Promary stain : carbofuchsin
Decollrizer: acid alchol
Counter stain: methlynr blue
Acid fast(red) not acid fast (blue)