Microbiology

Question 1

The Joshua and Esther Lederberg’s replica plating technique demonstrated that: streptomycin revealed the presence of –

Answer 1

streptomycin resistant bacteria

Question 2

T/F: In the lab, E. coli is grown at 37 degrees Celsius because it is a temperature sensitive mutant

Answer 2

true

Question 3

F- plasmid that can integrate into the bacterial chromosome by homologous recombination

Answer 3

episome

Question 4

Integration of the phage DNA into the bacterial chromosome is facilitated by – encoded in the host genome

Answer 4

DNA pol

Question 5

Binding to the enzyme’s active site is characteristic of an – inhibitor

Answer 5

irreversible competitive

Question 6

Irreversible because it forms– bond that creates a strong and permanent attachment

Answer 6

covalent

Question 7

– inhibitors utilize weak molecular attachment (i.e. van der Waals or hydrogen bonds) when attaching to the enzyme active site

Answer 7

reversible competitive

Question 8

penicillin’s effect is to inhibit the bacteria’s ability to – or prevent progeny from forming a necessary cell wall to counter osmotic pressure

Answer 8

remodel their cell wall

Question 9

noncompetitive inhibitors bind to an –

Answer 9

allosteric site

Question 10

binding ot a molecule to the allosteric site of an enzyme causes a conformational which affect the – of the active site

Answer 10

shape and function

Question 11

T/F: viruses can contain either DNA or RNA

Answer 11

true

Question 12

viruses genetical material is not arranged into a chromosome but is associated as a complex of –

Answer 12

nucleic acids and histone proteins

Question 13

an operon is a functional unit of genomic DNA containing a cluster of genes under the control of a –

Answer 13

single regulatory signal or promoter

Question 14

T/F: the genes contained in an operon are either expressed together or not at all

Answer 14

true

Question 15

several genes must be both – and co-regulated to be defined as an operon

Answer 15

co-transcribed