Microbiology Flashcards
Viruses are obligate intracellular parasite. what does that mean?
they are only able to reproduce within cells
it relies on host machinery
T/F
Viruses on their own are able to synthesis ATP and other chemical reactions.
False
T/F
Viruses are not cells or living organisms.
True
How can some viruses have ATP stored in their capsid? What can they use it for?
they got it from the last host
they can use it for penetration into the next host
When a virus is not inside a host cell, what does it contain?
only its own genome which could be single stranded or double stranded, linear or circular
If the ratio of adenine to thymine in a DNA virus is not one to one, what can be said about the genome of this virus?
the genome must be single stranded DNA, or RNA which has no T
A disease agent that is isolated from a human cannot reproduce on its own, but it can reproduce when put in a culture of human cells. In its pure form it contains both RNA and DNA, is it a virus? Why?
no because a virus can only have DNA or RNA but not both
What is a bacteriophage?
virus that infects bacteria
What limiting factor affects all viral genomes?
size
What happens if a viral genome triples in size?
it will no longer fit within the normal viral structure and therefore will not be packaged in infectious viral particles
How does the virus adapt to its size constraint?
- carries only a few genes and relies mostly on host encoded proteins for transcription, translation, and replication
- ability to encode more than one protein in a given length of genome by utilizing more than one reading frame within a piece of DNA so that genes overlap
How do ribosomes used to translate viral proteins compare to host ribosomes?
they are the same because viruses use the host ribosomes
What is the protein coat that surrounds the viral nucleic acid genome which is also used to classify viruses?
capsid
Helical capsids?
rod shaped
Polyhedral capsids?
multiple sided geometric figures with regular surfaces
What is the capsid shape of Bacteriophage T4?
Helical and Polyhedral
What is the host for the Bacteriophage T4?
E. coli
Where on the capsid is the genome located?
in the capsid head
What do the tail fibers and the base plate of the capsid do?
attach to the surface of the host cell
What does the sheath of the capsid do?
contracts using energy of stored ATP, injects the genome into the host
Why might a bacteriophage inject its DNA, while animal viruses do not?
a phage must puncture the bacterial cell wall, while animal viruses can be internalized whole because there is no cell wall
The entire viral capsid is composed of _______ while the viral genome is composed of ______.
proteins
nucleic acids
In animal viruses, what surrounds the capsid?
the envelope
What is the envelope?
membrane on the exterior of the animal virus derived from the host membrane
How do animal viruses acquire an envelope?
budding
What are viruses without an envelope?
naked viruses
-plant viruses and phages are all naked because they do not bud through the membrane, they puncture the cell wall
T/F
Viruses are very specific, not random.
True
What is the first step in infection?
virus binds to a specific receptor on the cell surface
-attachment or adsorption
After binding, how does the virus become internalized?
fusion with the plasma membrane or receptor mediated endocytosis
-penetration(animal) or eclipse(plant, bacteria)
If antibodies to a viral capsid protein are ineffective in blocking infection, what might this indicate about the virus?
the virus is enveloped, so the antibody cannot reach its epitope on the capsid surface
In the Lytic cycle, what happens as soon as the phage genome has entered the host cell?
host polymerases and ribosomes begin to rapidly transcribe and translate it
What is one of the first viral gene products made in the lytic cycle?
hydrolase- degrades entire host genome
Early genes?
a group of genes that are expressed immediately after infection and which includes any special enzymes required to express viral genes (ex. hydrolase)
What happens after hydrolase degrades the host genome in lytic cycle?
multiple copies of the phage genome are produced (using dNTPs resulting from degradation) as well as capsid proteins
What happens after copies of the phage genome are produced in the lytic cycle?
each new capsid assembles itself around a new genome
What happens after the new capsids form in the lytic cycle?
enzyme called lysozyme (late gene) is produced which destroys the bacterial cell wall, the host bursts (lyses) releasing hundreds of the new capsids to repeat the cycle
If lysozyme were an early gene, would this be advantageous to the virus?
no the host would burst before the phage had time to replicate and assemble
When phage are first added to a bacterial culture, the number of infective viruses initially decrease before it later increases, why?
many phage have injected their genomes into host cells and are no longer infectious, then the cells lyse and release a bunch of capsids
Bacteria culture in the presence of S-cysteine and P-phosphate are infected with T4, what will be found in the interior of newly infected bacteria?
only P because nucleic acids contain no S and proteins contain no P, when the virus infects bacteria their nucleic acids are injected while the proteins remain on the outside
A bacteriophage with an important capsid gene deleted infects the same cell as another virus (co-infection) with a normal copy of the same gene. What will happen at the time of lysis?
all released viruses will be capable of infecting new hosts, but only some of these new infections will give rise to phage capable of infecting new hosts.
some of the bad genome will be covered by good capsid, which can infect another cell but once in the cell the bad genome will code for bad capsid and its progeny will not be able to infect a host
Advantage and disadvantage of the lytic cycle?
- efficient way for a virus to rapidly increase its numbers
- all host cells are destroyed, eventually all will run out
In the lysogenic cycle, what happens after infection of the host?
the phage genome is incorporated into the bacterial genome and is now referred to as a prophage, host is called a lysogen
What is a prophage in lysogenic cycle?
silent, genes are not expressed, viral progeny not produced
Why is the prophage dormant in lysogenic cycle?
transcription of phage genes is blocked by a phage encoded repressor protein that binds to specific DNA elements in phage promoters (operators)
Why is the lysogenic cycle clever?
every time the host reproduces itself, the prophage is reproduced too
What happens when the prophage becomes activated in the lysogenic cycle?
removes itself from the host genome (excision) and enters the lytic cycle
What is a potential consequence of the lysogenic cycle?
when the viral genome activates, excising itself from the host genome, it may take part of the host genome with it and could integrate that DNA into future infections (transduction)
Why would a bacterial gene, carried with a virus and integrated with viral genes into a new bacterial genome, not be repressed along with the viral genes during lysogeny?
viral repressor proteins bind to a specific sequence on viral DNA, this sequence is not present on the bacterial gene, so the bacterial gene can be expressed while the viral genes are repressed.
What does the virus bind to on animal cells?
animal cells have proteins on the surface of the plasma membrane that serve as specific receptors for viruses
What is one of the first steps in HIV infection?
HIV virus protein gp120 binds to a T cell membrane protein called CD4
Would treatment of an HIV infected person with a soluble form of CD4 protein affect the infectivity of the virus?
yes, the CD4 protein would bind the virus CD4 receptors and block attachment of the virus to the T cells
Mutation of the cell surface receptor that viruses attach to would be a means for an organism to become resistant to viral infections. Why is this mechanism not common?
- the receptor has a specific role in the normal function of the host, which might be compromised
- viruses can evolve rapidly to keep up with the changes of the host
What is the next step in the infection of the animal cell after the virus has been bound to the plasma membrane?
penetration- many animal viruses enter by endocytosis
Endocytosis in animal virus infections?
the host cell engulfs the virus and internalizes it
Once inside the host cell, the viral genome becomes ______.
uncoated (released from capsid)
What are the three possible cycles of an animal virus?
lytic
productive
lysogenic
What is the difference between the productive and lytic cycles?
productive doesnt destroy the cell
How is the cell not destroyed in the productive cycle?
the enveloped virus exits the host by budding through the host’s cell membrane, the cell membrane can reseal itself so the cell doesnt die
What is a provirus in animal cells?
the dormant form of the viral genome in the animal virus lysogenic cycle
What is an example of a provirus in animal cells?
Herpes Simplex I is the virus that causes oral herpes
it can remain dormant until stress reactivates it
What is + RNA?
a piece of single stranded viral RNA which serves as mRNA
What happens when a + RNA virus in injected into the cell?
it acts as mRNA and host ribosomes immediately begin to translate it, creating viral proteins
Infective?
injecting an isolated genome into the host cell will result in virus production (+RNA)
What protein is needed for +RNA virus to replicate itself?
RNA dependent RNA polymerase
What is the role of RNA dependent RNA pol?
to copy the RNA genome for viral replication
What are some examples of +RNA viruses?
common cold, polio, rubella
If a viral genome is + strand RNA, what is used as a template by the RNA dependent RNA pol?
it needs the - strand RNA as a template so it first produces a - strand intermediate before generating new + strand genomes
What happens when a -RNA virus enters the cell?
it codes for and carries the RNA dependent RNA pol, unlike +RNA viruses, so when it enters the cell the enzyme creates a + strand from the - strand, then the cycle can continue
What are some examples of -RNA viruses?
rabies, measles, mumps, flu
What is a retrovirus?
+RNA viruses that undergo lysogeny
How can a retrovirus integrate into double stranded DNA genome if it enters the cell as RNA?
they undergo reverse transcription to make DNA from an RNA template, this is done by RNA dependent DNA polymerase (reverse transcriptase) encoded by the viral genome
Even though retroviruses carry the enzyme, RNA dependent DNA pol, they are theoretically not required to carry it, why?
the viral genome can be translated by host ribosomes so it only needs to encode for the enzyme
What are the three main retroviral genes and what do they code for?
gag- codes for viral capsid proteins
pol- codes for reverse transcriptase
env- codes for viral envelope proteins
After integration of a retrovirus into the cellular genome, a reverse transcriptase inhibitor is added to the cell, will the production of new viruses be blocked?
no because the reverse transcriptase is only required for the copying of the viral RNA genome into DNA, this will have already taken place so it is not needed anymore, so an inhibitor wouldnt block anything
What do double stranded DNA viruses often have?
large genomes that include genes for enzymes involved in dNTP synthesis and DNA replication
Why do double stranded DNA viruses carry around genes for enzymes that are possessed by the host cell?
if the virus wants to reproduce, it doesnt have to wait for the host cell to do it
Why dont RNA viruses carry the genes for dNTP synthesis or replication?
transcription is always occurring in all cells, so NTPs (not dNTPs) are always present
What factor likely limits the size of RNA genomes?
the error rate is much higher in RNA synthesis as opposed to DNA synthesis, so if the RNA genome was too large every copy would suffer too many errors that no infectious virus would be produced
What is the advantage for DNA viruses to induce host cell division?
to produce the ingredients needed for DNA synthesis if it does not already possess them
What is a virus that complements another virus?
helper virus (one virus provides one thing necessary, the other virus provides the other thing necessary, ex. adenoviruses)
What is the primary feature that distinguishes prokaryotes from eukaryotes?
prok do not have membrane bound organelles
What are three examples of prokaryotes?
bacteria
archea (extremophiles)
blue green algae (cyanobacteria)
Taxonomy?
classification of living organisms
What are the three recognized domains of taxonomy?
bacteria
archea
Eukarya
Domains can be subdivided into what?
kingdoms
What are the three Euk kingdoms?
Animalia
Plantae
Fungi