Microbiology Flashcards
What is the vector for yellow fever?
mosquitos (aedes aegypti)
The yellow fever virus belongs to which class of virsuses?
flaviviridae
What geographical locations are at risk for yellow fever?
Africa, central and south america, and the carribean
What is the vector for the “jungle” yellow fever, from the Haemagogus virus?
Monkeys to humans
What is the clinical manifestation why we call yellow fever “yellow?”
Jaundice
After 5 days, what are the coagulation defects that occur in yellow fever?
Prothrombin deficiency –> hematemesis and melena
What is the type of vaccine available for yellow fever?
Live attenuated 17D yellow fever vaccine
What is the vector for Dengue fever?
Mosquito
What geographical locations does Dengue fever exist?
SE asia
Pacific
India
South and central america
What is the mosquito that is the principal human vector for the transmission of Dengue fever?
A. aegypti
In which cells does Dengue fever replicate?
Monocytes
In addition to a flu-like illness, what skin conditon can occur in Dengue fever?
Maculopapular/erythmatous rash
This is the type of Dengue fever disease where there is vascular damage, shock, and hemorrhage everywhere.
Dengue Hemorrhagic fever (DHF)
What reinfection must occur to cause DHF?
reinfection with a different serotype than the original infection
Reinfection froma different serotype in DHF causes what molecules to bind to the virus and enchance its ability to infect monocytes?
Antibodies from the previous virus infection
This is the virus spread by the Aedes aegypti mosquito that causes an illness similar to Dengue but polyarthritis is very common and retro-orbital pain is rare.
Chikungunya virus
Where in the world is Chikungunya virus?
Africa
Asia
(there was an outbreak in Italy in 2007)
This is the encephalitis virus that is ssRNA, + stranded, enveloped, icosahedral nucleocapsid, found in the Atlantic gulf states of the USA, and is spread by Aedes mosquitos.
Eastern equine encephalitis (alphavirus)
EEE
This is the encephalitis virus that is ssRNA, + stranded, enveloped, icosahedral nucleocapsid, found west of the mississippi the USA and is spread by Culex mosquitos.
Western equine encephalitis (alphavirus)
This is the encephalitis virus that is ssRNA, + stranded, enveloped, icosahedral nucleocapsid, found in Africa, Europe, central asia, and USA, and is spread by Culex mosquitos.
West nile encephalitis (flavivirus)
This is the encephalitis virus that is ssRNA, + stranded, enveloped, icosahedral nucleocapsid, found in Southern, Central, and Western states, and is spread by the Culex mosquitos.
St. louis encephalitis (flavivirus)
characterisitcs look a lot like WEE
This is the encephalitis virus that is ssRNA, NEGATIVE stranded, enveloped, helic nucleocapsid, found in Northern and central USA states, and is spread by the Aedes mosquitos.
California enephalitis (bunyavirus)
This is the encephalitis virus that is ssRNA, + stranded, enveloped, icosahedral nucleocapsid, found in Far East and South East Asia, and is spread by Culex mosquitos.
Japanese encephalitis (flavivirus)
This is the encephalitis virus that is ssRNA, + stranded, enveloped, icosahedral nucleocapsid, found in Australia, and is spread by Culex mosquitos.
Murray valley encephalitis (flavivirus)
This is the encephalitis virus that is ssRNA, + stranded, enveloped, icosahedral nucleocapsid, found in Eastern europe, and is spread by a TICK.
Tick-borne encephalitis.
This is the encephalitis virus that is ssRNA, + stranded, enveloped, icosahedral nucleocapsid, found in Southern USA, central and south america, and is spread by mosquitos.
Venezuelan encephalitis (alpha virus)
This is the encephalitis virus that is ssRNA, + stranded, enveloped, icosahedral nucleocapsid, found in USA and canada, and is spread by ticks.
Powassan (flavivirus)
Yellow fever, Dengue, Kyasnur forest, Ross river, Rift valley fever, Sandfly fever, Congo-crimean hemorrhagic fever, Colorago tick fever, and La cross all have what in common?
They’re all Arboviruses
All arbovirsuses are ssRNA, +stranded and are enveloped except which one? What’s different about it?
Colorado tick fever
dsRNA, NON-enveloped
Yellow fever, Dengue, Kyasnur forest, Ross river, and Colorado tick fever have what type of nucleocapsid?
Icosahedral nucleocapsid
so what is the nucelocapsid of Rift valley fever, Congo-crimean hemorrhagic fever, and La cross?
helical nueleocapsid
Where is Kyasnur forest (flavivirus)? Vector?
India
tick
Where is Ross river (alphavirus)? Vector?
Australia, pacific islands
mosquito
Where is rift valley fever (bunyavirus)? Vector?
Aftrica
mosquito
Where is Sandfly fever (bunyavirus)? Vector?
Asia, south america, mediterranean
sandlflies
Where is Congo-Crimean hemorrhagive fever (bunyavirus)? Vector?
Asia, africa
tick
Where is La crosse (bunyavirus)? Vector?
USA
mosquito
This is the disease from:
Organism- R. rickettsii
Vector- tick
Reservoir- ticks, wild rodents
Rocky mountain spotted fever
This is the disease from:
Organism- R. akari
Vector- Mite
Reservoir- mites, wild rodents
Rickettsialpox
This is the disease from:
Organism- O. tsutsugamushi
Vector- mite
Reservoir- mites, wild rodents
Scrub typhus
This is the disease from:
Organism- R. prowazekii
Vector- Louse
Reservoir- Humans, squirrel fleas, FLYING SQUIRRELS
Epidemic typhus
This is the disease from:
Organism- R. typhi
Vector- Flea
Reservoir- Wild rodents
Murine typhus
This is the disease from:
Organism- E. chaffeensis and E. ewingii
Vector- Tick
Reservoir- Deer, small mammals
Ehrlichiosis
This is the disease from:
Organism- A. phagocytophilum
Vector- tick
Reservoir- deer, small mammals
Anaplasmosis
This is the disease from:
Organism- C. burnetii
Vector- none
Reservoir- cattle, sheep, goats, cats
Q fever
This is the test for Rickettsial disease that causes antibodies to the rickettsiae to cross-react with the O antigen polysaccharide of various strains of Proteus vulgaris.
Weil-Felix agglutination test
What is the DOC for rickettsial disease?
tetracyclines (eg doxycycline)
You shouldnt give tetracyclines to kids <9 y/o unless they have which rickettsial disease?
RMSF
What is the DOC for rickettsial diseases in pregnant women?
Chloramphenicol
What is the path of the rash in RMSF?
inwards: starts on the palms/soles and spreads towards the torso
What is the path of the rash in epidemic typhus (r. prowazekii)?
outward: torso –> extremities
Epidemic typhus is spread by R. prowazekii and is associated with what?
Poverty and war cuz it’s spread by lice
In addition to the outward spreading rash in Acute epidemic typhus, what is the Sx at 1 week?
Flu-like Sx
Why is there a high mortality rate in acute epidemic typhus?
due to peripheral vascular collapse or secondary bacterial PNA
What are the neurological manifestations of acute epidemic typhus?
Severe meningoencephalitis with delirium and coma
This is the type of epidemic typhus where there rickettsiae are not eliminated from the body, allowing the infection to reactivate as much as 50 years later.
Convalescent Epidemic typhus
Brill-Zinnser disease
What is the G- spirochete to cause epidemic (louse-borne) relapsing fever?
Borrelia recurrentis
After the bacteria multiple in the louse, they enter the body through a would when what happens in Epidemic louse-borne relapsing fever?
when the louse bites are rubbed and the lice are crushed.
wash yer hands!
What is the mortality rate in Epidemic louse-borne relapsing fever?
40%
What is the vector for ENdemic relapsing fever?
Tick bites
For Endemic (tick-borne) relapsing fever, what is the reservoir?
Rodents
The soft ticks in Endemic (tick-borne) relapsing fever are of what genus (think rustic cabins out West with mice).
Ornithodoros
What is the mortality rate of Endemic (tick-borne) relapsing fever?
< 5%
Which type of relapsing fever (epidemic or endemic) is this indicative of:
repeated febrile episodes ( week of incubation, 3-5 days of fever, wk of afebrile, etc) due to antigenic variation in the spirochetes
Trick question. Both epidemic and endemic cause the same Sx.
How many relapsing episodes are there usually in relapsing fever?
3-10
True or False: each relapsing episode in relapsing fever are less severe than the previous.
True!
Though the body makes Ab’s against Borrelia to fight the infection, what happens to the bacteria to cause the relapsing fever?
Antigenic variation of the plasmids
What is the test for relapsing fever for the Dx?
Giemsa-stained blood smears taken during the febrile period
What is the DOC for relapsing fever?
Tetracycline
What is this disease?
Vector- Ixodes ticks
Reservoir- rodents and deer
Organism- B. burdorferi
Lyme disease
What are the early clinical manifestations of Lyme disease after 1 week?
Fever, headache, myalgia, lymphadenopathy and Erythema migrans at the bite.
What happens to the erythema migrans in the late clinical manifestation of Lyme disease?
Enlarges but reamins red and flat with clear center
What are the neurological, cardiac, and rheumatological late clinical manifestations of Lyme disease?
Neuro- meningitis, enchephalitis, and peripheral neuropathy
Cardiac- heart block and myocarditis
Rheum- arthralgia and arthritis with immune comollexes for months-years.
What is the medium for the Dx of Lyme disease in the early-stage?
NSK medium
Why can’t you culture Lyme disease in the late stage, so you have to base it off of clinical presentation?
It can’t been seen during the late stage.
What is the specific assay test u can do for the Dx of Lyme disease?
ELISA with Western blot
Which Ab’s are detected 3-6 weeks after infection and which at a later stage for Lyme disease?
IgM at 3-6 and IgG later
What are the 2 DOC for early Lyme disease?
Doxycylcine or Amoxicillin
An IV of what antibiotic for 30 days will be needed for the Tx of late Lyme disease?
Ceftriaxone
What are the WBC’s affected by Ehrlichia and Anaplasma?
Granulocytes, monocytes, erythrocytes, and platelets.
What is the disaease caused by Ehrlichiosis?
Human monocytic Ehrlichiosis (E. chaffeensis)
What are the WBC’s affected by Ehrlichosis?
Monocytes
What are the Sx of Ehrlichiosis after 1-3 weeks?
flu-like Sx with high fever, headache, malaise, and myalgias with possible rash
What are the blood labs like after 1-3 weeks in Ehrlichiosis?
Leukopenia, thrombocytopenia, and elevated serum transaminases
What are the diagnostic methods of choice for Ehrlichiosis?
Serology and DNA probe tests
What is the DOC for Ehrlichiosis?
Doxycycline
What is the disease that works kinda like Ehrlichiosis but infects GRANULOCYTES instead of monocytes?
Anaplasmosis
What are the Sx of Anaplasmosis 5-11 days after exposure?
Flu-like illness with high fever, headache, malaise and myalgia (same as ehrlichiosis)
Why is Anaplasmosis more severe? What happens to macrophages?
Macrophages are activated
True or False: Anaplasmosis and Ehrlichiosis have the same diagnostic and treatment methods.
True
Matching! Match 1-4 with A-D. What are the following vectors for each disease?
A. Rickettsial
B. Lyme disease
C. Babesiosis
D. Ehrlichiosis/anaplasmosis
- Inhalation, consuming milk
- Hard ticks
- Amblyyomma americanum and Ixodes species
- Ixodid ticks
1-A
2-B
3-D
4-C
Match 1-4 with A-D. What are the following reservoirs for each disease?
A. Rickettsial
B. Lyme disease
C. Babesiosis
D. Ehrlichiosis/anaplasmosis
- Animals (deep, cattle, and rodents)
- White footed mouse and white tailed deer
- White tailed deer, white footed mouse, dogs, foxes, coyotes, and wolves
- Mammals, birds, and ticks
1-C
2-B
3-D
4-A
Match 1-4 with A-D. What are the following Sx/conditions for each disease?
A. Rickettsial
B. Lyme disease
C. Babesiosis
D. Ehrlichiosis/anaplasmosis
- Q fever
- Relapsing fever and erythema migrans
- General malaise, fever w/o periodicy, progress to renal failure/hepatomegaly,
- Monocytic and granulocytic disease
- A
- B
- C
- D
Match 1-4 with A-D. What are the following diagnosis methods for each disease?
A. Rickettsial
B. Lyme disease
C. Babesiosis
D. Ehrlichiosis/anaplasmosis
- Serology and DNA probe
- Isolation of organism or Dx levels of IgM or IgG.
- Serology by detecting Ab response to phase I and phase II Ag
- Exam blood smear-method of choice and serology tests
- D
- B
- A
- C
True or False: you begin to start to feel Sx of general malaise during the pre-erythocyte stage of Plasmodium infections.
False.
This stage is symptomless
After the sporozoites are injected from the mosquito saliva and into the blood, where do they mature for a couple weeks?
Parenchymal cells of the liver
After maturing in the liver, what are they called as the Plasmodium re-enters the bloodstream?
Merozoites
Some Plasmodium remains dormant as hypozoites, which can lead to what problem of malaria?
Relapses
In order to mature during the asexual blood stage, the merozoites enter the RBC and then turn into what to make more merozoites?
Trophozotes –> schizont –> merozoites
In the sexual stage, what do merozoites turn into so they can be taken up by the mosquito again?
Male and female gametocytes
Give the path of gametocytes –> sporozoites again in the mosquito.
Gametocytes enter the gut –> male gametocytes exglagellates –> male fertilizes female gametocytes –> zygote –> invasion of the gut mucosa –> oocyst –> sporozoites –> salivary glands of the mosquito.
What are the 4 genetic conditions where u can have resistance to malaria?
Duffy Ag (P. vivax resistance)
HbS
B-thallassemia
G6PD defeciency
What are the Sx to malaria infections following rupture of erythocytic schizonts?
Fluctuating fever and drenching sweats,
What strain of malaria shows 72 hours of periotic fever, where the others show 48?
P. malariae
Which malarial strains are self-limiting if infection doesnt occur?
P. vivax, ovale, and maleriae
How often are the fevers in P falciparum?
Daily (in the evening)
What are the systemic problems with malarial infections?
Hepatosplenomegaly, anemia, jaundice
Which malarial strain is the most virulent and kills u in the first 2 weeks?
P. falciparum
Which Plasmodia strain can cause nephrotic syndrome?
P. malaria
Which Plasmodium strain is severe in kids and pregnant chicks, and can cause cerebral malaria, szrs, coma, anemia, lactic acidosis and acute renal failure with acute tubular necrosis?
P. falciparium
What is the DOC for malaria?
IV artesunate
Quinolones are contraindicated in what pts?
G6PD deficiency pts
This is the form of malaria when they mature in the lung during the preerythrocytic stage.
Schizont
This is the form of malaria after the schizont mature and rupture into the blood stream, during the preerythrocytic stage, and they are what makes the ring formation.
Merozoite
This is the form of malaria that only happens in P. ovale and vivax, where they lie dormant in the liver to initiate the asexual blood stage.
Hypnozoite
This is the form of malaria when the merozoite matures within the RBC and then initiates the sexual stage, which is taken up by the mosquito.
Gametocytes
Since hyponozoites (literally “sleeping-ozoites”) lie dormant in the liver, what is the DOC to kill hypnozoites?
Primaquine
What is the vector for babesiosis?
Tick
After an infected tick bites human, what bodies are introduced into the bloodstrema nd infects the RBC’s for babesiosis?
Priform bodies
In babesiosis, the intraerythrocytic trophozoites multiply by binary fission, and then get released as what cells?
Merosoites
What is the DOC for babesiosis?
Clindamycin w/quinine
Which trypanosomiasis (African or American) does this belong under?
Causitive agent- trypanosoma brucei gambience and T.b. Rhodesiense
African
Which trypanosomiasis (African or American) does this belong under?
Vector- reduviid bug
American
Which trypanosomiasis (African or American) does this belong under?
Causitive agent- T. cruzi
American
Which trypanosomiasis (African or American) does this belong under?
Clinical features- Sleeping sickness (lymphadenopathy, fever, splenomegaly, CNS dysfxn, coma)
African
Which trypanosomiasis (African or American) does this belong under?
Vector- Tsetse fly
Afterican
Which trypanosomiasis (African or American) does this belong under?
Clinical features- chagas disease (macrophage invasian, chancres, invasion of muscle cells, affects heart and GI)
American
Which trypanosomiasis (African or American) does this belong under?
Dx- looking at the parasite in the blood film, serology or xenodiagnosis, PCR.
American
Which trypanosomiasis (African or American) does this belong under?
Treatment- Oral nifurtimox or benznidazole. Posaconazole
American
Which trypanosomiasis (African or American) does this belong under?
Dx- looking at parasite in blood, lymph nodes or CSF. Detection of antitrypanosomal Ab to screen.
African
What is the treatment for EAST African trypanosomiasis?
IV suramin followed by IV melarsoprol
What is the treatment for WEST African trypanosomiasis?
IM pentamidine or IV eflornithine
What is the vector for Leishmaniasis?
Sandflies
L. donoviani, infantum and chagasi belong to which category of leishmaniasis?
Visceral
L. major, tropica, aethiopica, mexicana, brazilensis, and peruviana belong to which category of leishmaniasis?
Cutaneous
Which form of leishmaniasis develops more slowly with fever and wt loss, followed by hepatosplenomegaly, and skin lesions may occur after treatment (PKDL)?
Visceral
Which form of leishmaniasis progresses insidiously from a small papule to a large ulcer, healing with scarring?
Cutaneous
What happens to the immunodeficient pt in response to leishmaniasis?
widespread chronic skin lesions and visceeral leishmaniasis
How do u Dx leishmaniasis via microscopy?
microscopy of splenic aspirate or bone marrow or skin lesion
Detection of which Ab via the direct agglutination test is good for leishmaniasis?
Antileishmanial Ab
What is the injected into the skin for cutaneous leishmaniasis?
Sodium stibogluconate
What is the DOC for visceral leishmaniasis?
Amphotericin B
What are the 2 causitive agents for lymphatic filariasis?
Brugia and Wucheria
What is the vector for lymphatic filariasis?
Mosquitos
What are the clinical features of filariasis?
fever, rashes, eosinophilia, ORCHITIS, elephantitis
What is the DOC for filariasis?
Ivermectin with albendazole
or
Albendazole + DEC
THis is the class of viruses that are enveloped, ssRNA, persist infection in their natural rodent hosts, and compose the Old world and New world diseases.
Arenviruses
What are the Old World (LCM-LASV complex) of arenaviruses?
Lassa fever, Lymphocytic Choriomeningitis
What are the new world arenaviruses?
Junin and machupoviruses
These are enveloped, - stranded, found in arthropod and rodents, and causes HFRS and HPS.
Bunyaviruses
These are long, filamentous, ssRNA viruses, have a fruit bat vector, and are composed of the Marburg and Ebola viruses?
Filoviruses
Why can’t C. burnetti be detected in blood cultures?
It’s intracellular
What are the 2 methods of Dx of Q fever?
PCR during the first week
IFA screening in complement fixing Ab titer
What forms on the skin in anthrax?
papules that ulcerate in their center and become black and necrotic
What is the Dx methods for anthrax?
Visualization of G+ rods
PCR assay
What is the DOC for anthrax?
Cipro
What does LF do to cells after PA delivers it ionto the cells?
Inhibits MAPKK –> apoptosis
What is the MOA of EF after PA delivers it to the cells?
increases cAMP –> edema
What are the virulence factors for the plague?
Antiphagocytic capsular Ag
Endotoxin
Protein toxins
What is the vector for the plague?
rat fleat
In the plauge, what may form in the armpit or groin?
bubos with hemorrhagic inflammation
What are the systemic complications with the plague?
fever, can spread to blood causing septicemia, hemorrhagic illness, and multisystem involvement.
What is teh DOC for the plague?
Streptomycin
What does Francisella tularensis require for growth?
Cysteine
What are the virulence factors for F. tularensis?
Antiphagocytic capsule
Hemolysin
Pilli
ABC proteins
Where does F. tularensis parasitize int eh body?
RES –> lives in macrophages
What are the cutaneous manifestations of F. tularensis?
skin ulcer (ulceroglandular tularemia) –> febrile illness and lymphatic spread –> painful lymph nodes
What is the agar for F. tularensis?
BCYE
What is the DOC for F. tularensis?
Streptomycin
This is a G- rod, nonmotile, nonsporulating, PCN-sensistive, oxidase + bacteria that is of normal flora of dogs and cats/
Pasturella multocida
What are the virulence factors for P. multocida?
Endotoxin
Capsule
LPS
What are the clinical manifestations of P. multocida?
Local- cellulitis and lumpadenitis
PNA and septicemia
What agars do P. multocida frow on?
blood and chocolate
NOT MACCONKEYS
What is the staining pattern of P. multocida?
Bipolar
like Y. pestis
What is the DOC for P. multocida?
Amoxicillin/clavulanate
This isa spirochete with hooked ends that is found in domestic mammals and is transmitted by ingestion of contaminated water or food.
Leptospira interrogans
What are the clinical manifestations of Leptospirosis?
flu-like illnesses that typically resolve, but can cause hepatitis, haundice, liver hemorrhage, uremia, bacteriuria, aseptic meningitis, conjunctival or scleral hemorrhage in CSF and aqueous humor
What product from rats has lots of Leptospirosis?
Their pee
This is a severe form of leptospirosis with hemorrhagic complication and kidney/liver failure.
Weil’s disease
What are the 2 DOC for Leptospirosis?
PCN and doxy
This is the disease from Spirillum minus (G- spiral) and Streptobacillus moniliformis (G- filamenous), and is from rat bites.
Rat bite fever
After 7-10 days, what are the Sx to rat bite fever?
fever, headache, and myalgia
Which bacteria of rat bite fever causes inflammed local lesion?
S. moniliformis
What are the heart and lung manifestations of rat bite fever?
Endocarditis and PNA
To Dx rat bite fever, u can culture S. monliniformis but not S. minus, so how do u see S. minus?
Dark field microscopy
like most spiral stuff
What are the 2 DOC for rat bite fever?
PCN and streoptomycin
these are G- nonmotile coccobacillu with intracellular replication, and are from animals.
Brucella spp
Which one of these Brocella spp is the most severe?
B. abortus, melintensis, suis, or canis
B. melintensis
Where are each Brucella spp from?
B. abortus- cows
B. melintensis- goats and sheep (esp their aborted fetuses)
B. suis- pigs
B. canis- dogs
Where do the Brucella travel to after they enter the blood?
Reticuloendothelial cells
After there are inflammatory rxns and central necrosis of the reticuloendothelial cells, what are the clinical manifestations?
gradual onset of malaise, fever, DRENCHING sweats, aching and weakness, and possible systemic infectionsk
What are the Dx methods for Brucellosis?
Culture (but takes up to 4 wks)
Agglutinin titers
What is the combo theroapy for up to 6 weeks for the Tx for brucellosis?
Tetracyclin and streptomycin
TMP-SMZ
What is the bacteria spp to cause trench fever and cat-scratch disease?
Bartonella spp
What are the lab characteristics of Bartonella spp?
G- aerobic rods with fastidious growth requirements
What is the Bartonella spp to cause trench fever?
B. quintana
What is the vector for B. quintana?
Lice
think lice spread in war trenches or homeless
In trench fever,t here is a severe headache, fever, weakness, and pain in the long bones at what time intervals?
5-day intervals.
What is the serology test for B. quintana?
Wild-Felix test
What is the DOC for B. quintana?
Oral erythromycin or doxy
What is the bartonella spp to cause cat scratch disease?
T. nugent
jk
B. henselae
What are the vectors for B. henselae?
cat and flea reservoirs
In addition to regional lymphadenopathy and granulomatous inflammation of the primary cut lesion, which organ is enlarged in cat scratch fever?
Spleen
What is teh DOC for cat scratch disease?
Azithromycin
THis is the condition caused by B. quintana or B. hensale in immunocompromised hosts and causes weird postules and crap all over the skin.
Bacillary angiomatosis
What is the heart manifestation of Bacillary angiomatosis?
Subacute endocardiits
What is the best method to Dx. Bartonella spp?
PCR