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Shifting Exams (MACHINES) > MICROBIOLOGY > Flashcards

MICROBIOLOGY Flashcards

1
Q

Gram Stain reagents

A

Primary Stain: Crystal Violet (1 min)
Mordant: Gram’s Iodine (1 min)
Decolorizer: Acetone Alcohol (Flow/Thin: 1-2 mins/Thick: 5-10 mins)
Counterstain: Safranin Red (30 sec)

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2
Q

Gram Positive result

A

Purple/Blue

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3
Q

Gram Negative color

A

Pink/red

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4
Q

ACID FAST STAIN reagents

A

Carbol Fuchsin - 30 mins
Acid Alcohol - thin: 1-2 mins, thick: 5-10 mins
Malachite Green - 1 minute

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5
Q

A prenatal test used to detect Group B strep in vagina/rectum of a pregnant woman

A

GBS SCREENING TEST

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6
Q

How rare can a GBS pass from pregnant woman to her fetus

A

1-2 cases/100

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7
Q

Principle of BACT/ALERT VIRTUO for Blood Culture

A

Colorimetric

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8
Q

Bottles for Blood Culture

A

Adult:
Green (aerobic) - 10 mL
Orange (anaerobic) - 10 mL

Pedia/Peds Plus:
Yellow - 1 to 3 mL

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9
Q

Agar for Salmonella and Shigella

A

Hektoen Agar

XLD

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10
Q

Tellurite Blood Agar

A

C. diphtheriae

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11
Q

Lowenstein-Jensen

A

M. tuberculosis

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12
Q

Agar for E. coli

A

EMB

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13
Q

Enterobacteriaceae

A

XLD

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14
Q

Buffered Charcoal Yeast Agar

A

Legionella pneumophilia

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15
Q

Legionella pneumophilia

A

Buffered Charcoal Yeast Agar

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16
Q

TCBS

A

V. cholerae

17
Q

V. cholerae

A

TCBS

18
Q

M. tuberculosis

A

Lowenstein-Jensen Agar

19
Q

Mannitol Salt Agar

A

S. aureus

20
Q

S. aureus

A

MSA

21
Q

TSI/BAP

A

Corynebacterium

22
Q

Corynebacterium

A

TSI/BAP

23
Q

Modified Thayer Martin

A

Neisseria

24
Q

Neisseria

A

Modified Thayer Martin

25
Q

EMB

A

E. coli

26
Q

Green Metallic Sheen

A

E. coli

27
Q

Bordet Gengou

A

Bordetella

28
Q

Bordetella

A

Bordet Gengou

29
Q

Fletcher Medium

A

Leptospira spp.

30
Q

Leptospira spp.

A

Fletcher Medium

31
Q

Cycloserine Cefloxin Fructose Agar

A

C. difficile

32
Q

C. difficile

A

Cycloserine Cefloxin Fructose Agar

33
Q

Haemophilus

A

CAP

34
Q

CAP

A

Haemophilus

35
Q

GeneXpert

A

RT-PCR

36
Q

VITEK 2

A

Identification: Biochemical

Sensitivity: MIC/Broth Microdilution MIC

37
Q

VITEK MALDITOF-MS

A

Matrix-Assisted Light Desorption Ionization - Time of Flight Mass Spectrometry

38
Q

BIOFIRE FILMARRAY (TORCH)

A

nested multiplex PCR

39
Q

BACT/ALERT VIRTUO

A

Colorimetry

Shifting Exams (MACHINES) (5 decks)

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