MICROBIOLOGY

Question 1

Gram Stain reagents

Answer 1

Primary Stain: Crystal Violet (1 min)
Mordant: Gram’s Iodine (1 min)
Decolorizer: Acetone Alcohol (Flow/Thin: 1-2 mins/Thick: 5-10 mins)
Counterstain: Safranin Red (30 sec)

Question 2

Gram Positive result

Answer 2

Purple/Blue

Question 3

Gram Negative color

Answer 3

Pink/red

Question 4

ACID FAST STAIN reagents

Answer 4

Carbol Fuchsin - 30 mins
Acid Alcohol - thin: 1-2 mins, thick: 5-10 mins
Malachite Green - 1 minute

Question 5

A prenatal test used to detect Group B strep in vagina/rectum of a pregnant woman

Answer 5

GBS SCREENING TEST

Question 6

How rare can a GBS pass from pregnant woman to her fetus

Answer 6

1-2 cases/100

Question 7

Principle of BACT/ALERT VIRTUO for Blood Culture

Answer 7

Colorimetric

Question 8

Bottles for Blood Culture

Answer 8

Adult:
Green (aerobic) - 10 mL
Orange (anaerobic) - 10 mL

Pedia/Peds Plus:
Yellow - 1 to 3 mL

Question 9

Agar for Salmonella and Shigella

Answer 9

Hektoen Agar

XLD

Question 10

Tellurite Blood Agar

Answer 10

C. diphtheriae

Question 11

Lowenstein-Jensen

Answer 11

M. tuberculosis

Question 12

Agar for E. coli

Answer 12

EMB

Question 13

Enterobacteriaceae

Answer 13

XLD

Question 14

Buffered Charcoal Yeast Agar

Answer 14

Legionella pneumophilia

Question 15

Legionella pneumophilia

Answer 15

Buffered Charcoal Yeast Agar

Question 16

TCBS

Answer 16

V. cholerae

Question 17

V. cholerae

Answer 17

TCBS

Question 18

M. tuberculosis

Answer 18

Lowenstein-Jensen Agar

Question 19

Mannitol Salt Agar

Answer 19

S. aureus

Question 20

S. aureus

Answer 20

MSA

Question 21

TSI/BAP

Answer 21

Corynebacterium

Question 22

Corynebacterium

Answer 22

TSI/BAP

Question 23

Modified Thayer Martin

Answer 23

Neisseria

Question 24

Neisseria

Answer 24

Modified Thayer Martin

Question 25

EMB

Answer 25

E. coli

Question 26

Green Metallic Sheen

Answer 26

E. coli

Question 27

Bordet Gengou

Answer 27

Bordetella

Question 28

Bordetella

Answer 28

Bordet Gengou

Question 29

Fletcher Medium

Answer 29

Leptospira spp.

Question 30

Leptospira spp.

Answer 30

Fletcher Medium

Question 31

Cycloserine Cefloxin Fructose Agar

Answer 31

C. difficile

Question 32

C. difficile

Answer 32

Cycloserine Cefloxin Fructose Agar

Question 33

Haemophilus

Answer 33

CAP

Question 34

CAP

Answer 34

Haemophilus

Question 35

GeneXpert

Answer 35

RT-PCR

Question 36

VITEK 2

Answer 36

Identification: Biochemical Sensitivity: MIC/Broth Microdilution MIC

Question 37

VITEK MALDITOF-MS

Answer 37

Matrix-Assisted Light Desorption Ionization - Time of Flight Mass Spectrometry

Question 38

BIOFIRE FILMARRAY (TORCH)

Answer 38

nested multiplex PCR

Question 39

BACT/ALERT VIRTUO

Answer 39

Colorimetry