CLINICAL CHEMISTRY Flashcards
Serum Samples and their tube Color
Routine Chem Tests (R/Y)
Random Blood Sugar
2 Post Prandial Blood Sugar
Oral Glucose Tolerance Test
Therapeutic Drug Monitoring (R)
Blood Alcohol (R)
Homocysteine (R)
Therapeutic Drug Monitoring
Cortisol
Vancomycin
Gentamicin
Digoxin
Valproic Acid
Methotrexate
Carbamazepine
Phenobarbital
Phenytoin
Plasma Samples (requires centrifugation)
lactate assay (gray)
Plasma ammonia (green)
NT-Pro BNP (green)
Troponin I (green - Lithium Heparin)
5 Tests that requires WHOLE BLOOD sample
HbA1c
Tacrolimus
Sirolimus
Cyclosporine
Troponin T
Tests that do not require centrifugation
WHOLE BLOOD samples such as:
Troponin T
HBA1C
CYCLOSPORINE
TACROLIMUS
SIROLIMUS
How many drops of HCl must be added to urine samples
2-3 drops and then invert tube 2-3 times also
Purpose of adding HCl in urine
Acidify and preserve samples
24 hr metabolic urine includes
Metanephrine
VMA
5-HIAA
Catecholamine
What specimen requires noting of bottle number
CSF
Ascitic/Peritoneal
Pleural
Pericardial
Routine 24 hr urine tests
Oxalate
Amylase
Calcium
Chloride
Magnesium
Phosphorus
Potassium
Sodium
Urea Nitrogen
Uric Acid
Glucose
Protein
Protein-Creatinine Ratio
Creatinine
Endogenous creatinine clearance
Send out to SLMC Global
24 hr metabolic urine: metanephrine, HIAA, VMA, Catecholamine
Glycomark (1,5 anhydroglucitol - R/Y)
Amikacin - Red top
Tests requiring RED TOP only
Therapeutic Drug Monitoring
Blood Alcohol
Homocysteine
Amikacin
HBA1C test (machine and principle)
ARKRAY - HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)
Sample and reagents for HBA1C
5 ul WHOLE blood
450 ul diluent 80
Serum and Random Urine Osmolality (machine and principle)
3320 Osmometer - Freezing Point Osmolality
Ionized Calcium (machine and principle)
NOVAPRIME - Ion Selective Electrode (ISE)
ATELLICA SCI principle in chemistry
Spectrophotometry
TDM, Tacrolimus, Sirolimus and Cyclosporine is done in what machine
ARCHITECT - Chemiluminiscence Microparticle Assay
Principle of Diazyme DZ Lite
Chemiluminescence Immunoassay
Adenosine Deaminase uses what machine
Diazyme DZ Lite
Specimen for Adenosine Deaminase (ADA)
Pleural, Ascitic, Pericardial, CSF
Centrifuge (rpm and mins)
4000 rpm
15 mins
Atellica DLC principle
Spectrophotometry
Tacrolimus measurement of sample and reagent
Tacro = 200 ul WB + 200 ul precip
Cyclosporine measurement of sample and reagent
Cyclo = 200 ul WB + 100 ul solubilization rgnt + 400 ul precipitation rgnt
Sirolimus measurement of sample and reagent
Siro = 150 ul WB + 300 ul precip rgnt
Microcentrifuge of tacrolimus
14000 rpm
4 mins
Microcentrifugation of cyclosporine and sirolimus
10,000 rpm
4 mins
