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Shifting Exams (MACHINES) > CLINICAL SEROLOGY AND IMMUNOLOGY > Flashcards

CLINICAL SEROLOGY AND IMMUNOLOGY Flashcards

1
Q

Colors of the tube that requires centrifugation:

A

Red top

Yellow top

Lavender top for Plasma Metanephrine

Lavender tube (in ice) for ACTH

Lavender tube for Chromogranin A

Green tube for Plasma Insulin

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2
Q

Tubes that do not need centrifugation

A

Blue (LAC)

Lavender Tube for Hgb Electrophoresis

Lavender tube for CMV IF Ag

Urine Chlamydia

Random and 24hr urine electrophoresis

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3
Q

Routine centrifugation of samples

A

4000 rpm for 15 mins

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4
Q

HCG-BETA

A

ATELLICA SCI

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5
Q

AFP

A

ATELLICA SCI

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6
Q

E2 (Estradiol)

A

ATELLICA SCI

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7
Q

TPSA

A

ATELLICA SCI

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8
Q

ATELLICA SCI principle

A

Chemiluminescence Immunoassay

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9
Q

FSH

A

ATELLICA SCI

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10
Q

LH

A

ATELLICA SCI

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11
Q

CEA

A

ATELLICA SCI

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12
Q

PTH

A

ATELLICA SCI

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13
Q

Vitamin D

A

ATELLICA SCI

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14
Q

CA 15-3

A

ATELLICA SCI

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15
Q

TSH

A

ATELLICA SCI

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16
Q

CA 19-9

A

ATELLICA SCI

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17
Q

PROG

A

ATELLICA SCI

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18
Q

FPSA

A

ATELLICA SCI

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19
Q

PRL

A

ATELLICA SCI

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20
Q

CALCT

A

ATELLICA SCI

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21
Q

T3 and T4

A

ATELLICA SCI

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22
Q

FT3 and FT4

A

ATELLICA SCI

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23
Q

TESTOSTERONE

A

ATELLICA SCI

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24
Q

Triiodothyronine

A

ATELLICA SCI (T3)

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25
Q

Thyroxine

A

ATELLICA SCI (T4)

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26
Q

HBsAg

A

ARCHITECT i2000SR

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27
Q

ARCHITECT i2000SR principle

A

CHEMILUMINESCENCE MICROPARTICLE IMMUNOASSAY

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28
Q

Anti-HBs

A

ARCHITECT i2000SR

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29
Q

HBeAg

A

ARCHITECT i2000SR

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30
Q

A-HBe

A

ARCHITECT i2000SR

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31
Q

HAV IgM2

A

ARCHITECT i2000SR

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32
Q

HAV IgG2

A

ARCHITECT i2000SR

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33
Q

A-HCV

A

ARCHITECT i2000SR

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34
Q

Core IgM

A

ARCHITECT i2000SR

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35
Q

Core Total

A

ARCHITECT i2000SR

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36
Q

Ferritin

A

ARCHITECT i2000SR

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37
Q

Insulin

A

ARCHITECT i2000SR

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38
Q

Vitamin B12

A

ARCHITECT i2000SR

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39
Q

Folate II

A

ARCHITECT i2000SR

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40
Q

C Peptide

A

ARCHITECT i2000SR

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41
Q

SHBG

A

ARCHITECT i2000SR

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42
Q

CA 125

A

ARCHITECT i2000SR

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43
Q

HE4

A

ARCHITECT i2000SR

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44
Q

SCC

A

ARCHITECT i2000SR

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45
Q

PEPSINOGEN I

A

ARCHITECT i2000SR

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46
Q

PEPSINOGEN II

A

ARCHITECT i2000SR

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47
Q

CYFRA 21-1

A

ARCHITECT i2000SR

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48
Q

PRO-GRP

A

ARCHITECT i2000SR

49
Q

DHEAS

A

ARCHITECT i2000SR

50
Q

PIVKA II

A

ARCHITECT i2000SR

51
Q

Principle of ACCESS 2

A

CLIA

52
Q

ACCESS 2 tests:

A

TG2

ATG

ATP

EPN

HGrH

IL

OSTASE

AMH

53
Q

Other name of OSTASE

A

BONE SPECIFIC ALKALINE PHOSPHATASE

54
Q

IMMAGE 800 Principle

A

Nephelometry and Turbidimetry

55
Q

CRPH

A

IMMAGE 800

56
Q

CRP

A

IMMAGE 800

57
Q

RF

A

IMMAGE 800

58
Q

ASO

A

IMMAGE 800

59
Q

IgGAME (IgG, IgA, IgM, IgE)

A

IMMAGE 800

60
Q

C3

A

IMMAGE 800

61
Q

C4

A

IMMAGE 800

62
Q

TRF

A

IMMAGE 800

63
Q

LPAX

A

IMMAGE 800

64
Q

AAT

A

IMMAGE 800

65
Q

PAB

A

IMMAGE 800

66
Q

CER

A

IMMAGE 800

67
Q

VIDAS 3 Principle

A

ENZYME LINKED FLUORESCENCE ASSAY (ELFA)

68
Q

Procalcitonin IgM

A

VIDAS 3

69
Q

HIV-5 IgM

A

VIDAS 3

70
Q

CMV IGM

A

VIDAS 3

71
Q

HAV Total

A

VIDAS 3

72
Q

RUB IGM AND IGG

A

VIDAS 3

73
Q

LYME IgM/G

A

VIDAS 3

74
Q

Toxo IgM/IgG

A

VIDAS 3

75
Q

COVID IgM/G

A

VIDAS 3

76
Q

Normal result for PCT in VIDAS

A

Less than 0.10 ng/mL

77
Q

Critical result of PCT in VIDAS

A

> 2.0 ng/mL

78
Q

ATELLICA DLC principle

A

Spectrophotometry

79
Q

Test done in ATELLICA DLC

A

Cystatin C

80
Q

ALEGRIA principle

A

ELISA (ENZYME LINKED IMMUNOSORBENT ASSAY)

81
Q

Soluble Ag + Soluble Ab = INSOLUBLE complexes

A

Precipitation

82
Q

Aggregation of particulate antigens to form larger complexes when a specific antibody is present

A

Agglutination

83
Q

OVERALL STRENGTH OF Ag-Ab BINDING and the sum of affinities of all individual ag-ab combining sites

A

Avidity

84
Q

Number of multivalent sites of Ag when Ab binds are approximately equal

A

ZONE OF EQUIVALENCE

85
Q

formed when antibody binds to more than one antigen (vice-versa)

A

LATTICE

86
Q

Antibody excess

A

PROZONE

87
Q

What to do when a prozone happened

A

Serial Dilution

88
Q

excess antigen

A

Postzone

89
Q

What to do when a postzone happened

A

Repeat test after 1 week

90
Q

Effect of prozone and postzone with the result

A

False NEGATIVE (-)

91
Q

Measures the cloudiness of a solution

A

Turbidity

92
Q

Rate of scattering increases as reagent antibody is added

A

Rate nephelometry

93
Q

Measures REDUCTION in LIGHT as it passes through a solution by REFLECTION, ABSORPTION, and SCATTER

A

Turbidity

94
Q

Measures cloudiness of a solution

A

TURBIDITY

95
Q

Turbidity is reported in what unit

A

Absorbance units

96
Q

amount of light scatter is proportional to the

A

Size, shape, and concentration of molecules

97
Q

Measures LIGHT SCATTERED at a particular angle (10°-90°) from incident beam as it passes throught a suspension

A

Nephelometry

98
Q

PASSIVE IMMUNODIFFUSION TECHNIQUES

A

Ag and Ab are added to wells and ag-ab combination occurs by DIFFUSION

99
Q

Single diffusion

A

Radial Immunodiffusion (RID)

100
Q

Antigen allowed to diffuse to completion

A

Endpoint (sq of dm is proportional to ag conc.)

101
Q

Uses measurements taken before point of equivalence is reqched

A

Dm is propor to log of conc.

102
Q

Ouchterlony Double Diffusion

A

Double Diffusion
Double Dimension

103
Q

Fusion of lines at their junction to form an arc

A

Serological identity

104
Q

Two separate reactions

A

Non-Identity

105
Q

Fusion of 2 lines with a spur

A

Partial Identity

106
Q

Used in detection of monoclonal gammopathies

A

Immunofixation Electrophoresis

107
Q

Western Blot

A

Immunofixation Electrophoresis

108
Q

Also known as AGGLUTINOGENS

A

Antigens

109
Q

Other name of antibodies

A

Agglutinins

110
Q

Also known as ANTIGENIC DETERMINANT

A

EPITOPE

111
Q

Ag-Ab combination through a single antigenic determinant

A

SENSITIZATION

112
Q

Particles COATED with ANTIGEN not normally found on their surface

A

Indirect/Passive Agglutination

113
Q

Antigen found naturally on a particle

A

Direct Agglutination

114
Q

ABO typing is an example of what agglutination

A

DIRECT

115
Q

Particles coated with Antibody

A

Reverse Passive Agglutination

116
Q

Lack of agglutination is the positive reaction

A

Agglutination Inhibition

117
Q

Uses bacteria as the inert particles to which antibody is attached

A

COAGGLUTINATION

118
Q

False Positive results (F+)

A

Overcentrifugation

Contaminated glasswares, slides, rgnt

Autoagglutination

Saline stored in glass bottles

Cross reactivity

Rheumatoid Factor

Heterophile Ab

Delay in reading a slide test

Shifting Exams (MACHINES) (5 decks)

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