CLINICAL SEROLOGY AND IMMUNOLOGY Flashcards
1
Q
Colors of the tube that requires centrifugation:
A
Red top
Yellow top
Lavender top for Plasma Metanephrine
Lavender tube (in ice) for ACTH
Lavender tube for Chromogranin A
Green tube for Plasma Insulin
2
Q
Tubes that do not need centrifugation
A
Blue (LAC)
Lavender Tube for Hgb Electrophoresis
Lavender tube for CMV IF Ag
Urine Chlamydia
Random and 24hr urine electrophoresis
3
Q
Routine centrifugation of samples
A
4000 rpm for 15 mins
4
Q
HCG-BETA
A
ATELLICA SCI
5
Q
AFP
A
ATELLICA SCI
6
Q
E2 (Estradiol)
A
ATELLICA SCI
7
Q
TPSA
A
ATELLICA SCI
8
Q
ATELLICA SCI principle
A
Chemiluminescence Immunoassay
9
Q
FSH
A
ATELLICA SCI
10
Q
LH
A
ATELLICA SCI
11
Q
CEA
A
ATELLICA SCI
12
Q
PTH
A
ATELLICA SCI
13
Q
Vitamin D
A
ATELLICA SCI
14
Q
CA 15-3
A
ATELLICA SCI
15
Q
TSH
A
ATELLICA SCI
16
Q
CA 19-9
A
ATELLICA SCI
17
Q
PROG
A
ATELLICA SCI
18
Q
FPSA
A
ATELLICA SCI
19
Q
PRL
A
ATELLICA SCI
20
Q
CALCT
A
ATELLICA SCI
21
Q
T3 and T4
A
ATELLICA SCI
22
Q
FT3 and FT4
A
ATELLICA SCI
23
Q
TESTOSTERONE
A
ATELLICA SCI
24
Q
Triiodothyronine
A
ATELLICA SCI (T3)
25
Thyroxine
ATELLICA SCI (T4)
26
HBsAg
ARCHITECT i2000SR
27
ARCHITECT i2000SR principle
CHEMILUMINESCENCE MICROPARTICLE IMMUNOASSAY
28
Anti-HBs
ARCHITECT i2000SR
29
HBeAg
ARCHITECT i2000SR
30
A-HBe
ARCHITECT i2000SR
31
HAV IgM2
ARCHITECT i2000SR
32
HAV IgG2
ARCHITECT i2000SR
33
A-HCV
ARCHITECT i2000SR
34
Core IgM
ARCHITECT i2000SR
35
Core Total
ARCHITECT i2000SR
36
Ferritin
ARCHITECT i2000SR
37
Insulin
ARCHITECT i2000SR
38
Vitamin B12
ARCHITECT i2000SR
39
Folate II
ARCHITECT i2000SR
40
C Peptide
ARCHITECT i2000SR
41
SHBG
ARCHITECT i2000SR
42
CA 125
ARCHITECT i2000SR
43
HE4
ARCHITECT i2000SR
44
SCC
ARCHITECT i2000SR
45
PEPSINOGEN I
ARCHITECT i2000SR
46
PEPSINOGEN II
ARCHITECT i2000SR
47
CYFRA 21-1
ARCHITECT i2000SR
48
PRO-GRP
ARCHITECT i2000SR
49
DHEAS
ARCHITECT i2000SR
50
PIVKA II
ARCHITECT i2000SR
51
Principle of ACCESS 2
CLIA
52
ACCESS 2 tests:
TG2
ATG
ATP
EPN
HGrH
IL
OSTASE
AMH
53
Other name of OSTASE
BONE SPECIFIC ALKALINE PHOSPHATASE
54
IMMAGE 800 Principle
Nephelometry and Turbidimetry
55
CRPH
IMMAGE 800
56
CRP
IMMAGE 800
57
RF
IMMAGE 800
58
ASO
IMMAGE 800
59
IgGAME (IgG, IgA, IgM, IgE)
IMMAGE 800
60
C3
IMMAGE 800
61
C4
IMMAGE 800
62
TRF
IMMAGE 800
63
LPAX
IMMAGE 800
64
AAT
IMMAGE 800
65
PAB
IMMAGE 800
66
CER
IMMAGE 800
67
VIDAS 3 Principle
ENZYME LINKED FLUORESCENCE ASSAY (ELFA)
68
Procalcitonin IgM
VIDAS 3
69
HIV-5 IgM
VIDAS 3
70
CMV IGM
VIDAS 3
71
HAV Total
VIDAS 3
72
RUB IGM AND IGG
VIDAS 3
73
LYME IgM/G
VIDAS 3
74
Toxo IgM/IgG
VIDAS 3
75
COVID IgM/G
VIDAS 3
76
Normal result for PCT in VIDAS
Less than 0.10 ng/mL
77
Critical result of PCT in VIDAS
>2.0 ng/mL
78
ATELLICA DLC principle
Spectrophotometry
79
Test done in ATELLICA DLC
Cystatin C
80
ALEGRIA principle
ELISA (ENZYME LINKED IMMUNOSORBENT ASSAY)
81
Soluble Ag + Soluble Ab = INSOLUBLE complexes
Precipitation
82
Aggregation of particulate antigens to form larger complexes when a specific antibody is present
Agglutination
83
OVERALL STRENGTH OF Ag-Ab BINDING and the sum of affinities of all individual ag-ab combining sites
Avidity
84
Number of multivalent sites of Ag when Ab binds are approximately equal
ZONE OF EQUIVALENCE
85
formed when antibody binds to more than one antigen (vice-versa)
LATTICE
86
Antibody excess
PROZONE
87
What to do when a prozone happened
Serial Dilution
88
excess antigen
Postzone
89
What to do when a postzone happened
Repeat test after 1 week
90
Effect of prozone and postzone with the result
False NEGATIVE (-)
91
Measures the cloudiness of a solution
Turbidity
92
Rate of scattering increases as reagent antibody is added
Rate nephelometry
93
Measures REDUCTION in LIGHT as it passes through a solution by REFLECTION, ABSORPTION, and SCATTER
Turbidity
94
Measures cloudiness of a solution
TURBIDITY
95
Turbidity is reported in what unit
Absorbance units
96
amount of light scatter is proportional to the
Size, shape, and concentration of molecules
97
Measures LIGHT SCATTERED at a particular angle (10°-90°) from incident beam as it passes throught a suspension
Nephelometry
98
PASSIVE IMMUNODIFFUSION TECHNIQUES
Ag and Ab are added to wells and ag-ab combination occurs by DIFFUSION
99
Single diffusion
Radial Immunodiffusion (RID)
100
Antigen allowed to diffuse to completion
Endpoint (sq of dm is proportional to ag conc.)
101
Uses measurements taken before point of equivalence is reqched
Dm is propor to log of conc.
102
Ouchterlony Double Diffusion
Double Diffusion
Double Dimension
103
Fusion of lines at their junction to form an arc
Serological identity
104
Two separate reactions
Non-Identity
105
Fusion of 2 lines with a spur
Partial Identity
106
Used in detection of monoclonal gammopathies
Immunofixation Electrophoresis
107
Western Blot
Immunofixation Electrophoresis
108
Also known as AGGLUTINOGENS
Antigens
109
Other name of antibodies
Agglutinins
110
Also known as ANTIGENIC DETERMINANT
EPITOPE
111
Ag-Ab combination through a single antigenic determinant
SENSITIZATION
112
Particles COATED with ANTIGEN not normally found on their surface
Indirect/Passive Agglutination
113
Antigen found naturally on a particle
Direct Agglutination
114
ABO typing is an example of what agglutination
DIRECT
115
Particles coated with Antibody
Reverse Passive Agglutination
116
Lack of agglutination is the positive reaction
Agglutination Inhibition
117
Uses bacteria as the inert particles to which antibody is attached
COAGGLUTINATION
118
False Positive results (F+)
Overcentrifugation
Contaminated glasswares, slides, rgnt
Autoagglutination
Saline stored in glass bottles
Cross reactivity
Rheumatoid Factor
Heterophile Ab
Delay in reading a slide test
