Microbiology

Question 1

Which group of bacteria is gram positive, cocci and catalase positive?
(Example)

Answer 1

Staphylococcus
(Staph aureus)

Question 2

Which group of bacteria is gram positive, cocci and catalase negative?
(Example)

Answer 2

Streptococcus
(Strep. epidermidis)

Question 3

What 2 tests separate catalase negative gram positive cocci?

Answer 3

Haemolytic test and Lancefield grouping

Question 4

Give an example of gram positive alpha-haemolytic cocci bacteria

Answer 4

Strep Pneumoniae
Strep Viridins

Question 5

Give an example of gram positive cocci that is beta-haemolytic

Answer 5

Strep. pyogenes

Question 6

Give an example of gram positive cocci bacteria that is gamma-haemolytic (stays red)

Answer 6

Enterococci

Question 7

What colour are the zones for alpha and beta haemolysis

Answer 7

Alpha (green)
Beta (clear)

Question 8

What is the first test/agar used on gram negative bacteria? What does it show?

Answer 8

MacConkey agar
(Lactose fermenting - red/pink)
(Non-lactose fermenting - yellow/transparent)

Question 9

What test separates non-lactose fermenting gram-negative bacteria?

Answer 9

Oxidase

Question 10

What bacteria is gram negative and turns red (positive/pink) on MacConkey agar

Answer 10

E. coli
Kleibsella

Question 11

What test/agar separates the oxidase negative gram negative bacteria?

Answer 11

XLD agar

Question 12

What bacteria is red with black centre on XLD agar?

Answer 12

Salmonella

Question 13

What bacteria is red only XLD agar?

Answer 13

Shigella

Question 14

What bacteria is responsible for wound infections + toxic shock syndrome?

Answer 14

Staph aureus

Question 15

What bacteria is responsible for throat and skin infections?

Answer 15

Strep pyogenes

Question 16

What bacteria is responsible for CAP?

Answer 16

Strep. pneumoniae

Question 17

What bacteria is responsible for dental infection and subsequent infective endocarditis?

Answer 17

Strep. Viridans

Question 18

Describe strep. viridans characteristics

Answer 18

Gram positive
Cocci
Catalase -ive
Alpha-haemolytic

Question 19

What conditions is E. coli responsible for?

Answer 19

UTIs
Gastroenteritis
Travellers diarrhoea

Question 20

What does a shigella infection cause?

Answer 20

Dysentery

Question 21

What conditons does salmonella infection cause?

Answer 21

• Salmonella enteritidis: common cause of food poisoning
• Salmonellaa typhi: faecal oral spread

Question 22

What does a Proteus Mirabilis infection cause?

Answer 22

UTIs
Kidney stones

Question 23

What does klebsiella pneumonia infection cause?

Answer 23

UTI
Pneumonia

Question 24

What condition does Vibrio Cholerae cause?

Answer 24

Cholera (severe diarrhoea)

Question 25

What complications can Helicobacter Pylori cause?

Answer 25

Peptic ulcers of GORD

Question 26

What is the bacteria is responsible for whooping cough?

Answer 26

Bordetella Pertussis

Question 27

What does Legionella bacteria cause?

Answer 27

Legionnaires disease Notifiable disease Cause of atypical pneumonia

Question 28

What does a campylobacter infection cause?

Answer 28

Food poisoning (BBQs) Guillian Barre

Question 29

What is Neisseria spp responsible for?

Answer 29

Meningitis Gonorrhoea