Microbial Genetics

Question 1

Sticking end cutting

Answer 1

Fragments with overhang

Question 2

Blunt end cutting

Answer 2

Fragments with no overhang

Question 3

Recombinant DNA technology

Answer 3

the artificial recombination of DNA from two organisms

Question 4

Plant Ti Plasmid

Answer 4

The Ti plasmid of Agrobacterium tumberfaciens is made into recombination DNA and then infects plant

Question 5

Genomic library

Answer 5

Record of genomic DNA fragments that contains a gene of interest

Question 6

Recombinant Phage DNA

Answer 6

Use bacterial phages to hold more foreign DNA compared to plasmids

Question 7

Ways to insert genetic info

Answer 7

Electroporation, microinjection

Question 8

In prokaryotes, reproduction is always _______

Answer 8

asexual

Question 9

Binary fission

Answer 9

Most common mechanism of cell replication, cell grows, increases cellular components and divides via cytokinesis

Question 10

DNA probe

Answer 10

Probes are complimentary with gene of interested and labelled with a molecular beacon

Question 11

Microassay analysis

Answer 11

Detects cancerous vs healthy cells using reverse trascriptase labelling