Microbial control Flashcards
free of life of every kind
Sterile
the process of complete removal or destruction of all forms of microbial life which may be effected by physical or chemical means
Sterilization
having the property of inhibiting bacterial growth or multiplication
▪ Bacteriostatic
having the property of killing or destroying bacteria
Bactericidal
the process of killing or removing microorganisms on inanimate surfaces through the use of chemical agents
Disinfection
a chemical substance used to kill infection- producing microorganisms on surfaces but too toxic to be applied directly to tissues
Germicide or Disinfectant
characterized by the presence of pathogenic microbes in living tissues
Septic
characterized by the absence of
pathogenic microbes
Aseptic
a chemical substance which opposes sepsis or putrefaction either by killing microorganisms or by preventing their growth; applied topically to living tissues
Antiseptic
that temperature which in a given time destroys all the bacteria present
Thermal Death Point
- minimum time required to kill a suspension of organisms at a given temperature in a specified environment
Thermal Death Time
Period of adaptation; little or no multiplication but active metabolically
Lag Phase (Phase of Rejuvenescence or
Phase of Physiologic Youth
Organisms grow at maximum rate
Logarithmic Phase (Exponential Phase)
Rate of cell reproduction equals the rate of cell death
Stationary Phase (Plateau Phase or
Phase of Equilibrium)
- Total number of viable cells in the population decreases as cells die off at a constant rate
Death Phase (Phase of Decline
▪ aka. Clean Technique
▪ involves procedures and practices that reduce the number and transmission of pathogens
Medical Asepsis
▪ aka. Sterile Technique
▪ includes practices used to render and keep objects and areas sterile (free from microorganisms)
Surgical Asepsis
Easy to obtain and inexpensive; rapid
evaporation limits their contact time
Alcohols
Capable of destroying all forms of microbial life. Irritating to the respiratory tract, skin, and eyes
Aldehydes
(Formaldehyde)
Relatively low toxicity, destroys a wide range of microbes, adheres to and persists on skin and mucous membranes
Biguanides(Chlorhexidine)
Easily penetrates hard- to- reach places and fabrics and does not damage moisturesensitive material. It is toxic, explosive, and potentially carcinogenic
Ethylene Oxide Gas
Chlorine solutions are inexpensive and readily available however, organic compounds and other impurities neutralize the activity. Iodine is more expensive than chlorine and does not reliably kill endospores
Halogens (Chlorine and Iodine)
Most metal compounds are too toxic to be used medically
Metals (Silver
This unstable form of molecular oxygen readily breaks down
Ozone
Readily biodegradable and less toxic than traditional alternatives. The effectiveness of hydrogen peroxide as an antiseptic is limited because the enzyme catalase breaks it down. Peracetic acid is more potent germicide than is hydrogen peroxide.
Peroxygens (hydrogen peroxide and peracetic acid)
Wide range of activity, reasonable cost, remains effective in the presence of detergents and organic contaminants, leaves an active antimicrobial residue
Phenolic Compounds (triclosan and hexachlorophene)