microbes in food and beverage industry pt 2 Flashcards

1
Q

whats biocontrol

A

A method of introducing an agent organism into a new ecosystem to naturally prey on and suppress target “pest” organisms.

agent = newly introduced

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2
Q

describe acid fermantation of dairy products

A

milk fermentation ebings with laciid acid fermentation with lactobacillus and streptococcus

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3
Q

acid fermentation of vegetables

A
  • many vegetable products based on microbial fermentation
    soybeans: microbes remove harmful elements, mold in rhizopus (tempeh) and asperhillus (miso, soy sauce)
  • cabbage, cucmbers and olives: pickling fermentation in brine (high salt) with bacterium eluconostoc mesenteroides
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4
Q

describe alkaline fermented foods

A
  • ferment protens
  • natto and pidan are products of alkaline fermentation
  • bacillus breaks fownt he ‘bad’ components of soybean to make mroe digetable - get a stringy mass of polyglutamic acid
  • Bacillus transforms duck eggs to gelatinous delicacy
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5
Q

describe fermentation of cocoabean to make chocoalte

A
  • no starter culture has been commercialized and fermentation must take place geographically close to harvesting
  • fermentation by indigenous microbes involves 3 successive stages
  • after fermentation and pulp drainage beans are dried and raosted
  • cocoa liquor and butter are extracted frmo beans and recombines with sugar and other stuff to make cocoamass
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6
Q

what are the 3 successive stages of cocoa fermentation

A
  1. anerobic fermentation by yeast
  2. anaerobic fermentation by lactib acid bacteria
  3. aerobic fermentation by acetobacter species
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7
Q

why cant dogs eat chcolate

A

contains theobromine and 2 phenylethylamine - psychoactive products of cocoa fermentation

  • dogs metabolize theobromine very slow and can be toxic to them
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8
Q

what is responsible for fermentation of bread and wine

A

ethanolic fermentation by saccharomyces cerevisiae (yeast)

  • convers pyruvate to ethanol and CO2, CO2 is the leavening agent in bread
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9
Q

yeast bread vs sourdough

A
  • bread dough is leavened by microbial CO2
  • yeast: requires extensive kneading of gluten rich flour to make network of holes for CO2 to be released

Sourdough: as well as bakers yeast uses a cutlure of undefined lactobacillus sp[

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10
Q

whats injera

A
  • ethiopian bread

bade by teff, grain with no gluten

  • dough requires prolonged fermentation with extensiev/complex microbial activity
  • makes an adible “clothe”
  • fermentation dominated by yeast candida
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11
Q

what micrbo used for beer

A

– saccharomyces cerevisiae

  • beer derives from alcoholic fermentation of grain
  • barley grains are germinated, allowing enzymes to break down the starch into maltose for yeast fermentation
  • secondary products like long chian alc and esters generate the flavours

**excess diacteyl gives off the flavours

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12
Q

what type of fermentation does wine undergo

A

malolactic by oenococcus oeni bacteria

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13
Q

what are causes food spoilage

A
  • microbial chanegs that render a product unit or unpalatable for consumption
  • actis -> sour taste

Oxidation of fats -> rancidity

Decomposition of proteins -> putrification

alklainity -> bitter taste

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14
Q

food contmaination vs intoxication

A

food contamination (food poisoning) refers to presence of human pathogens

food intoxication refers to the ignestion of toxins in food (produced from/by bacteria growing in food)

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15
Q

how do the following spoil: dairy, meat, seafood, plants

A

Dairy: soured by excessive fermentation or made bitter by bacterial proteolysis

meat: putrefied by decarboxylating bacteria producing amines with noxious odors
seafoods: spoil rapidly bc of unsaturated fatty acids rapidly oxidize

*psychotrophic bacteria reduce trimethylamine oxidase to the fishy smelling trimethyl amine

  • plants spoil by excessive bacterial or mold gorth- pathogens can destroy food crops ebfore harvest
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16
Q

what is aspergillus flavus

A
  • aflatoxin: carcinogen
17
Q

what happens whn you se a can has a dent

A
  • foos intoxication
  • anaerobic, toxigenic endospore forming gram pos bacteria clostridium botulinum

*Botulism

18
Q

what is listeria monocytogenes

A
  • psychotropic bacterium that is ubiquitus int he environment
  • invades the intestinal epithelium causing listeriosis (complications for fetus)
  • can grow in fridge
  • minly rpoblem in unpasteurizes milk products liek cheeses
19
Q

describe intoxication and an example

A
  • most dangerous consequence of infection by food brone pathogens is toxin productin
  • clostridium botulinum toxin inhibits fusion of synpatic vesicles thus prevening activation of muscle cells
  • causes flaccid paralysis
20
Q

what are prebiotics, what are the two types

A
  • ‘food’ for gut microbes: typically non digestible fibre compounds but microbes can digest

*not one size fits all but marketed that way

Short chain

  • fermentation site = ascening colon
  • contain 2-8 links per saccharide molecule
  • ex: oligofructose (FOS)

Long chain

  • preferred fermentaion site = descending colon
  • 9-64 links/ saccharide mol
  • insulin
  • found in pany plants - typically roots or rhizomes
21
Q

what are full spectrum prebiotics

A
  • fermentaion ocurs throughout colon and usually mixtues of short and long chian prebiotics
  • ed: oligofructose enriched insulin
22
Q

what are synbiotics

A
  • pre and pro biotics
  • indeally probiotic strain encapsulated with a prebiotic which it likes to emtabolize
23
Q

what is a prebiotic

A

the food industry would have u think prebiotics are beneficial bc they fertilize the good microbes of the gut

  • prebiotics are also metabolized by potentially harmful microbes
  • can cause diarrhea, bloating and gas
  • nothing wrong with suign them but be objective