Microarray

Question 1

Gene expression definition

Answer 1

The level of activity of genes in a cell

Question 2

What kind of genes are up regulated

Answer 2

Proto-oncogenes

Question 3

What kind of genes are down regulated

Answer 3

Tumor suppressors/p53

Question 4

What does a microarray do

Answer 4

Compares gene expression of cells / jeans

Question 5

What is in each well of a microarray chip

Answer 5

Multiple copies of the same strand of DNA from the same gene

Question 6

Hybridized definition

Answer 6

Find

Question 7

What happens when DNA hybridizes to the DNA on a microarray well

Answer 7

Binds to any well with complementary strand

Question 8

Red meaning

Answer 8

Expressed more in cancer cells/upregulated

Question 9

What does green mean

Answer 9

Expressed more and healthy cells/down regulated

Question 10

What does yellow mean

Answer 10

Expressed equally in both / unaffected

Question 11

What does black mean

Answer 11

Gene is turned off / not express

Question 12

Purpose of Pearson correlation coefficient

Answer 12

Measures relationship between two sets of data

Question 13

Limitations for DNA microarray

Answer 13

Expensive and time consuming, similar sequences May bind or hybridize to the wrong spot, not all MRA that is produced gets translated into a protein

Question 14

Meaning of ratios

Answer 14

> 1: upregulated
<1: down regulated
=1: not affected
0: not expressed

Question 15

Meaning of ratios in base two logs

Answer 15

+: upregulated
- : down regulated
1: not affected

Question 16

Correlation coefficient meaning

Answer 16

+: Gene profiles behave similarly, the larger and closer to one means a stronger relationship
- means behave opposite, farther from 0 is stronger
1 means exactly the same
0 means unrelated