DNA Sequencing Notes

Question 1

Short sequence of DNA that are complementary to the Target sequence

Answer 1

Primer

Question 2

What does PCR stand for

Answer 2

Polymerase chain reaction

Question 3

Machine that copies DNA

Answer 3

Thermal cycler

Question 4

Enzyme that copies the template strand

Answer 4

DNA polymerase

Question 5

Steps to PCR

Answer 5

Break hydrogen bonds then primers attach. Then DNA polymerase copies the template

Question 6

Cytosine

Answer 6

Blue

Question 7

Thymine

Answer 7

Green

Question 8

Adenine

Answer 8

Red

Question 9

Guanine

Answer 9

Yellow/Black

Question 10

How to remember nucleotide pairs

Answer 10

Apple tree car garage

Question 11

How does cycle sequencing work

Answer 11

Randomly added fluorescently tagged nucleotides Mark the end of a segment and tells DNA polymerase to stop copying.

Question 12

How do we read the bases in cycle sequencing

Answer 12

DNA runs through an electrophoresis tube and a laser reads the marked nucleotides and spits out a chart with color coordinated loops

Question 13

PCR versus cycle sequencing

Answer 13

PCR: millions of copies that are all the same order of bases and length
Cycle sequencing: fluorescent nucleotides are used and fragments are different lengths and patterns

Question 14

E Value

Answer 14

Expected value or chance of a random match should be close to zero or negative

Question 15

Percent identity

Answer 15

Percent of entered sequence that match sequence should be 98 to 100